ACTIONS OF MAMMALIAN AMACRINE CELLS

拟议研究的长期目标是了解 哺乳动物视网膜的内部环路塑造感受野和 视网膜神经节细胞的反应特性。 具体目标是完成对兔视网膜主要成分的普查 细胞群。哺乳动物的视网膜很早就知道了 包含许多单元格类型和子类型。尽管我们不太可能 分析所有视网膜的罕见细胞，我们相信我们是 现在能够识别和绘制所有主要的地图。 最初的问题是：水平、两极、 无长突细胞呢？这个问题将由两个独立的 一系列的实验。在第一种情况下，视网膜细胞的数量将是 通过染色顺序鉴定。随处可见的污渍， 荧光标记和抗体将被用来区分个体 单元格类别。在第二种情况下，视网膜细胞的三大类 将通过共聚焦显微镜进行区分。 每种细胞类型在视网膜表面的分布如下 定量测绘。 这些实验将(首次)提供估计的 无长突细胞总数。通过将该数字与 组织化学染色的无长突细胞总数 和放射自显影的方法，我们会知道有多少不明的无长突 单元格必须仍然存在。然后，未知单元格的形状将是 通过向无长突细胞注射路西法的实验寻找 黄色。注射剂将与荧光、组织化学或 用放射自显影标记细胞体以获得种群密度 注射的细胞。

The long-term objective of the proposed research is to understand how the internal circuits of mammalian retinas shape the receptive fields and response properties of the retinal ganglion cells. The specific aim is to complete the census of the rabbit retina's major cell populations. Mammalian retinas have been known for a long time to contain many cell types and subtypes. Although it is unlikely that we can analyze all of the retina's rare (infrequent) cells, we believe that we are now able to identify and map all of the major ones. The initial question is: what are the total numbers of horizonal, bipolar, and amacrine cells? The question will be approached by two independent sets of experiments. In the first, populations of retinal cells will be sequentially identified by staining. Generally available stains, fluorescent labels and antibodies will be used to distinguish individual classes of cell. In the second, the three major classes of retinal cells will be distinguished by confocal microscopy. The distribution of each cell type across the retinal surface will be mapped quantitatively. These experiments will make available (for the first time) an estimate of the total number of amacrine cells. By comparing that number with the total numbers of the amacrine cells that have been stained by histochemical and autoradiographic methods, we will learn how many unidentified amacrine cells must still exist. The shapes of the unidentified cells will then be sought by experiments in which amacrine cells are injected with lucifer yellow. Injection will be combined with fluorescent, histochemical or autoradiographic labeling of cell bodies to give the population densities of the injected cells.