MECHANISM SOF RECURRENCE IN ANTERIOR UVEITIS OF THE EYE

男性特发性前葡萄膜炎的特点是 血-房水屏障是一种急性且经常复发的疾病。听之任之， 它经常会导致继发性青光眼。由于目前可用 眼部炎症的客观测量通常需要不受欢迎的方法 侵入性手术，如穿刺术，临床评估 前葡萄膜炎的严重程度及其对治疗的反应取决于 严重依赖虹膜充血、红斑等参数的主观分级 和细胞。拟议研究的一个主要目的是使 非侵入性目标的水基荧光分光光度技术 测量眼前节炎症。最终直接、临床 该程序的应用是我们的长期目标。以下是初步报告 在兔的研究中，水相荧光光度法将用于相关 志贺氏菌内毒素诱导的前葡萄膜炎动物模型的研究 猴子。通过利用最近可用的荧光素标记 超微结构示踪剂，我们将能够关联荧光光度 前葡萄膜炎严重程度的评估 对同一只眼睛进行超微结构示踪研究。其他研究，使用 上述相关程序，结合冷冻骨折 技术，将考察重建的过程 前葡萄膜炎消退过程中的血-房水屏障 不使用类固醇和非类固醇抗炎治疗 探员们。将特别强调以下机制： 构成解剖等价物的紧密连接的重组 血-房水屏障。障碍改革不完整或有缺陷， 葡萄膜炎后，可能会导致复发。因此，了解 壁垒改革的过程可能对理解 男性复发性前葡萄膜炎。对该病病因的进一步洞察 复发性前葡萄膜炎将由电子显微镜和 冷冻骨折研究比较猴子诱发疾病模型和 一种在马身上自然发生的模型，称为周期性马眼炎。

Idiopathic anterior uveitis in man is characterized by a breakdown of the blood-aqueous barrier which is acute and often recurrent. Left untreated, it can frequently lead to secondary glaucoma. Since currently available objective measures of ocular flammation usually necessitate undesirable invasive procedures such as paracentesis, clinical assessment of the severity of anterior uveitis, and its response to therapy, has relied heavily upon subjective grading of such parameters as iris hyperemia, flare and cells. A major aim of the proposed studies is adaptation of the technique of aqueous fluorophotometry to the objective, but non-invasive measure of anterior segment inflammation. Eventual direct, clinical application of the procedure is our long-range goal. Following preliminary studies in rabbits, aqueous fluorophotometry will be used for correlative studies of a Shigella endotoxin-induced model of anterior uveitis in monkeys. By taking advantage of recently available fluorescein-labelled ultrastructural tracers, we will be able to correlate fluorophotometric estimates of the severity of anterior uveitis with results of ultrastructural tracer studies on the same eyes. Additional studies, using the above correlative procedures, in conjunction with the freeze-fracture technique, will examine the process of re-establishment of the blood-aqueous barrier during resolution of anterior uveitis, both with and without treatment using steroidal and non-steroidal anti-inflammatory agents. Particular emphasis will be given to the mechanisms involved in the reassembly of tight junction, which constitute the anatomic equivalent of the blood-aqueous barrier. Incomplete or faulty barrier reformation, after uveitis, could contribute to recurrence. Thus, knowledge of the process of barrier reformation may be critical to an understanding of recurrent anterior uveitis in man. Further insights into the etiology of recurrent anterior uveitis will result from electron microscopic and freeze-fracture studies comparing the induced disease model in monkeys with a naturally occurring model in horses termed periodic equine ophthalmia.