ENDOTHELIUM MITOGEN IN RETINA--STRUCTURE AND APPLICATION

该项目的目标是通过两个阶段的研究来指导 其最终目标是开发抑制视网膜色素变性的方法， 新生血管形成 本建议（第一阶段）的范围集中于 特定血管的分子结构研究 视网膜内皮细胞有丝分裂原。 具体目标包括：净化 和来自血管内皮细胞的特异性血管内皮细胞有丝分裂原的表征。 胎牛视网膜 这项研究如果成功，将为制备提供化学基础 内皮细胞有丝分裂原类似物和内皮细胞有丝分裂原抗体在 今后（第二阶段）将进行一些涉及该机制的调查 血管内皮细胞增殖和视网膜 新生血管形成 血管内皮细胞增殖是主要的 视网膜新生血管形成过程中的事件。 有关调查 与血管内皮细胞增殖可以独立进行 如果不知道视网膜的精确机制， 新生血管形成过程。 与涉及整个过程的程序不同 视网膜新生血管的研究，血管内皮细胞实验 扩散的范围更窄。 此外，该实验 这种方法将使研究人员能够更有效地设计他们的 实验和解释他们的数据。 此外，目前的成功 该提案可能会为这一及时的调查提出新的方法。 我们和其他实验室的研究表明， 内皮细胞有丝分裂原是一种蛋白质。 因此，这个实验 项目基本上是在我们目前的知识范围内， 蛋白质化学 纯化实验将包括： 高效液相色谱法;薄层肽图谱;和 离子交换、金属螯合亲和性和肝素亲和性 色谱法。 将分析纯化的肽的金属离子 含量、氨基酸组成、N-和C-末端和分子量。 如果可行，将进行肽序列测定。

The objective of this project is directed through two stages of studies with an ultimate goal of developing methods of inhibiting retinal neovascularization. The scope of the present proposal (Stage I) focuses on investigation of the molecular structure of the specific vascular endothelium mitogens in retina. The specific aims include: purification and characterization of the specific vascular endothelium mitogen(s) from fetal bovine retina. The study, if successful, will provide a chemical basis for the preparation of endothelium mitogen analogs and endothelium mitogen antibodies in the future (Stage II) for a number of investigations dealing with the mechanism of vascular endothelial proliferation and inhibition of retinal neovascularization. Vascular endothelial proliferation is one of the major events in the retinal neovascularization process. Investigations concerned with vascular endothelial proliferation can be performed independently without knowledge of the precise mechanism of the retinal neovascularization process. Unlike programs involving the entire process of retinal neovascularization, experiments on vascular endothelial proliferation are more narrowly focused. In addition, this experimental approach will enable investigators to more effectively design their experiments and interpret their data. Furthermore, success of the present proposal may advance new approaches to this timely investigation. Studies in our and other laboratories indicate that the vascular endothelium mitogen(s) is a protein. Therefore, the experiments of this project are essentially within the scope of our present knowledge of protein chemistry. The purification experiments will include: high performance liquid chromatography; thin-layer peptide mapping; and ion-exchange, metal chelate affinity, and heparin affinity chromatographies. The purified peptide will be analyzed for metal ion content, amino acid composition, N- and C- termini, and molecular weight. Peptide sequence will be performed, if feasible.