ACTIVATION OF THE HEXOSEMONOHOSPHATE SHUNT IN THE LENS

己糖一磷酸分流(HMPS)及其相关物质的研究 在这一应用中提出了路径。我们将检查以下内容 五个主题：1)HMPS的控制；2)HMPS及其 相关途径；3)多元醇途径的重新检查和以前 未识别的速率限制步骤；4)干扰对 膜代谢；5)氧化对基础HMPS活性的影响 和抗氧化性。 这些研究是基于过去两年获得的数据。我们有 发现的证据：1)醛糖还原酶与NADPH的竞争 谷胱甘肽还原酶和2)膜代谢产物的显著变化 糖尿病大鼠。我们将采用生化程序以及 生物物理方法，如核磁共振波谱 研究不同的NADPH途径与电子的相互作用 顺磁共振波谱探测膜结构的变化。 这些研究对于理解糖尿病是至关重要的。 白内障的发生。研究抗氧化性可以为下一步工作奠定基础 用于设计非手术抗老年性白内障的治疗方法。

Studies on the hexose monophosphate shunt (HMPS) and its associated pathways are proposed in this application. We will examine the following five topics: 1) Control of the HMPS; 2) Activity of the HMPS and its associated pathways; 3) Re-examination of the polyol pathway and previously unrecognized rate-limiting steps; 4) The significance of disturbance to membrane metabolism; and 5) The effect of oxidation on basal HMPS activity and oxidative resistance. These studies are based on data obtained over the past two years. We have found evidence of: 1) competition for NADPH between aldose reductase and glutathione reductase and 2) prominent changes in membrane metabolites in the diabetic rats. We will employ biochemical procedures as well as biophysical methods such as nuclear magnetic resonance spectroscopy to examine the interaction of various NADPH-requiring pathways and electron paramagnetic resonance spectroscopy to probe membrane structural change. These studies are crucial to the understanding of diabetic cataractogenesis. Studies on oxidative resistance can pave the groundwork for the design of non-surgical anti-senile cataract therapy.