ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

这项建议的长期目标是调查 与水状体的产生有关的细胞机制 睫状上皮 具体目标是体外电生理学 纤毛离体制剂转运过程的研究 上皮 这些实验将提供关于正常的 生理机制和药理学诱导的改变涉及 在积极生产的房水。 房水减少 通过药物治疗形成是一种常见的治疗方法， 与青光眼相关的眼内压。 相关知识 负责房水产生的细胞机制将提供 了解房水形成的正常速率和可能的 降低房水率的药理学机制 生产 将分离的虹膜-睫状体制备物安装在腔室中 用于测量跨上皮电参数（电势 差、短路电流、组织电阻）、净流体 传输和单向通量（离子、水、非电解质）。 这些研究代表了主动运输和流体的巩固 组织制备中的血流维持正常的解剖结构 睫状体双层上皮之间的关系 流程. 常规微量移液管穿刺研究用于测量 膜电位将与 经上皮测量。 这些研究将提供以下信息： 单个睫状上皮细胞与离子运动的关系， 运输系统。 电生理检查将用于 研究下列各类药物：（一）卤化吸入剂 麻醉剂，（ii）肾上腺素能激动剂（iia） 神经肽Y与肾上腺素能激动剂，（iii）巴比妥类，和（iv） 叠氮化钠 这些实验将提供有关机制的信息 参与积极生产的水状体，并可能导致新的 和独特的抗青光眼药物， 在房水产生中的连续步骤。

The long-term objective of this proposal is the investigation of cellular mechanisms relating to the production of aqueous humor by the ciliary epithelium. Specific aims are the in vitro electrophysiologic study of transport processes on isolated preparations of the ciliary epithelium. These experiments will furnish information on normal physiological mechanisms and pharmacological-induced alterations involved in the active production of aqueous humor. Reduction of aqueous humor formation by medical therapy is a frequent treatment of the elevated intraocular pressure associated with glaucoma. Knowledge regarding cellular mechanisms responsible for aqueous humor production will provide insights into the normal rate of aqueous humor formation and possible pharmacologic mechanisms for the reduction in the rate of aqueous humor production. Isolated iris-ciliary body preparations are mounted in chambers for measurement of transepithelial electrical parameters (potential difference, short-circuit current, tissue resistance), net fluid transport, and unidirectional fluxes (ionic, water, non-electrolyte). These studies represent a consolidation of active transport and fluid flow in a tissue preparation maintaining the normal anatomic relationships between the double epithelial layers of the ciliary processes. Conventional micropipet impalement studies for measurement of membrane potential will be performed in conjunction with the transepithelial measurements. These studies will provide information on individual ciliary epithelial cells in relationship to ion movements and transport systems. Electrophysiologic investigations will be used to study the following classes of drugs: (i) halogenated inhalation anesthetic agents, (ii) adrenergic agonists (iia) the interaction of neuropeptide Y with the adrenergic agonist, (iii) barbiturates, and (iv) sodium azide. These experiments will furnish information on mechanisms involved in the active production of aqueous humor and may result in new and unique antiglaucoma agents designed to inhibit the different sequential steps in aqueous humor production.