ACTIONS OF MAMMALIAN AMACRINE CELLS

拟议研究的长期目标是了解 哺乳动物视网膜的内部回路形成感受野， 视网膜神经节细胞的反应特性。 具体目的是完成对家兔视网膜的普查 细胞群 很久以前就知道哺乳动物的视网膜 包含许多细胞类型和亚型。 尽管我们不太可能 分析所有的视网膜的罕见（罕见）细胞，我们相信，我们是 现在能够识别并绘制所有主要的地图。 最初的问题是：双相，双相， 和无长突细胞 这个问题将由两个独立的 几组实验。 首先，视网膜细胞群将被 通过染色依次鉴定。 一般可用的染色剂， 荧光标记和抗体将用于区分个体 类细胞。 第二种是视网膜细胞的三种主要类型 将通过共聚焦显微镜进行区分。 每种细胞类型在视网膜表面的分布将是 量化映射。 这些实验将（首次）提供 无长突细胞的总数。 通过将这个数字与 组织化学染色的无长突细胞总数 和放射自显影的方法，我们将了解有多少不明的无长突 细胞必须存在。 然后，未识别细胞的形状将被 在实验中，无长突细胞被注入路西法， 黄色的. 注射将与荧光、组织化学或 放射自显影标记细胞体以给出群体密度 注射的细胞。

The long-term objective of the proposed research is to understand how the internal circuits of mammalian retinas shape the receptive fields and response properties of the retinal ganglion cells. The specific aim is to complete the census of the rabbit retina's major cell populations. Mammalian retinas have been known for a long time to contain many cell types and subtypes. Although it is unlikely that we can analyze all of the retina's rare (infrequent) cells, we believe that we are now able to identify and map all of the major ones. The initial question is: what are the total numbers of horizonal, bipolar, and amacrine cells? The question will be approached by two independent sets of experiments. In the first, populations of retinal cells will be sequentially identified by staining. Generally available stains, fluorescent labels and antibodies will be used to distinguish individual classes of cell. In the second, the three major classes of retinal cells will be distinguished by confocal microscopy. The distribution of each cell type across the retinal surface will be mapped quantitatively. These experiments will make available (for the first time) an estimate of the total number of amacrine cells. By comparing that number with the total numbers of the amacrine cells that have been stained by histochemical and autoradiographic methods, we will learn how many unidentified amacrine cells must still exist. The shapes of the unidentified cells will then be sought by experiments in which amacrine cells are injected with lucifer yellow. Injection will be combined with fluorescent, histochemical or autoradiographic labeling of cell bodies to give the population densities of the injected cells.