ELECTROPHYSIOLOGY OF CILIARY BODY TRANSPORT PROCESSES

房水的形成主要是由房水中的活性分泌物 双层睫状突上皮(内层无色素和外层 有色素的上皮细胞)和超滤(在有 静水压力)。最终的房水成分由以下因素决定 这两种行为都受到许多因素的影响，包括 全身和局部代谢改变、体液影响、循环 变化和神经功能的改变。这项建议的目的是 分离的虹膜睫状体(I-CB)转运过程的研究 不受这些因素的影响。实验技术将包括 以下1)I-CB组织制备中的跨上皮测量 电位差、短路电流、组织电阻、离子 通量和流体传输提供主动传输的概述 睫状体上皮中的突起。2)常规离子比和pH值 微电极研究离子转移的步骤 穿过睫状突的双上皮层。3) 膜片钳对无色素细胞层内细胞膜的研究。 在体跨上皮、细胞内微电极和膜片钳 微吸管测量可以提供对正常生理的洞察 和睫状上皮的主动运输。药理研究 将提供有关睫状体上皮的基本和改变状态的信息 分泌和运输过程。被调查的代理人包括 肾上腺素能激动剂和拮抗剂，碳酸氢酶抑制剂，钙 通道阻滞剂和神经肽(例如，抗利尿激素， 血管活性肠多肽等)。机制方面的知识 参与房水的主动产生及其影响 通过睫状体上皮主动转运的药理药物， 将提供针对青光眼治疗方式的见解 减少房水形成的速度。

Aqueous humor formation is mainly a composite of active secretion by the double layered ciliary process epithelium (inner nonpigmented and outer pigmented epithelium) and of ultrafiltration (dialysis in the presence of hydrostatic pressure). Final aqueous humor composition is determined by both of these actions which are influenced a number of factors including systemic and local metabolic alterations, humoral influences, circulatory changes and neurological modifications. The aim of this proposal is to investigate transport processes in the isolate iris-ciliary body (I-CB) independent of these factors. Experimental techniques will include the following 1) Transepithelial measurement in the I-CB preparation of tissue potential difference, short-circuit current, tissue resistance, ionic fluxes and fluid transport to provide an overview of active transport processes in the ciliary epithelium. 2) Conventional ionic specific and pH microelectrode studies to characterize the steps in ion translocation across the double epithelial layer of the ciliary processes. 3) Patch-clamp studies on the inner cell membrane of the nonpigmented layer. In virto transepithelial, intracellular microelectrode, and patch-clamp micropipette measurements can provide insights into the normal physiology and active transport of the ciliary epithelium. Pharmacological studies will provide information on basic and altered states of ciliary epithelial secretory and transport processes. Agents to be investigated include adrenergic agonists and antagonists, carbonic anhydrase inhibitors, calcium channel blocking agents, and neuropeptides (e.g. anti-diuretic hormone, vasoactive intestinal polypeptide, etc). Knowledge of the mechanisms involved in the active production of aqueous humor, as well as the effects of pharmacological agents on active transport by the ciliary epithelium, will provide insights into glaucoma therapeutic modalities directed to reducing the rate of aqueous humor formation.