EFFECT OF LIGHT ON THE OCULAR IMMUNE RESPONSE

描述(改编自申请人的摘要)：注射抗原 眼睛的前房(AC)导致独特的免疫模式 反应称为前房相关免疫偏离(ACAID)， 哪些抗原特异性抑制T细胞(TS细胞)抑制全身性 迟发型超敏反应(DTH)。通过使用暗饲养和光饲养 小鼠(Balb/c)以前证明可见光有一个 直接影响这种眼内免疫反应。如果光线被阻挡 从通过暗饲养到达眼睛，通过将光饲养的动物放在 深色后的交流注射，或通过闭上眼皮的光养 动物在黑暗中注射交流电后，或通过闭上眼皮 轻养动物后交流注射抑制被取消。此外， 研究表明，可见光而不是紫外线或红外光是 对观察到的影响负责。 光的影响是一种眼部事件，不是发育过程中的。 视觉发育是强制性的，但在黑暗长大的成年人中是可以诱导的 动物。这些结果表明，眼内存在活跃的T细胞。 在没有光线的情况下防止压制的规则。目的 这一提议的目的是进一步定义细胞和分子事件 可见光对眼内免疫反应的影响。申请人 将确定导致抑制的潜伏期的波长 以获得可能反映 分子介体在效应中的作用。他将决定是否改变 明/暗条件改变了人眼的分布和表达 I类、II类和其他与免疫反应相关的分子 (CD4、Thy-1、LCA)。他将分析以下诱导的调节细胞 交流注射和从眼睛移除光线，因为这些细胞是 能够阻断T细胞的活性。进一步的研究将会 确定氧活性化合物、神经递质和 神经肽在眼内免疫的明暗调节中的作用 反应。调查人员认为，这些研究具有重要的意义 对理解眼内免疫反应的意义以及可能 眼科疾病的治疗。

DESCRIPTION (adapted from applicant's abstract): Injection antigen into the anterior chamber (AC) of the eye results in a unique pattern of immune responses known as Anterior Chamber Associated Immune Deviation (ACAID), in which antigen-specific suppressor T-cells (Ts-cells) inhibit systemic delayed type hypersensitivity (DTH). By using dark-reared and light-reared mice (Balb/c) it was previously demonstrated that visible light has a direct effect on this intraocular immune reaction. If light is prevented from reaching the eye by dark-rearing, by placing light reared animals in the dark post AC injection, or by closing the eyelid of light-reared animals in the dark post AC injection, or by closing the eyelid of light-reared animals post AC injection suppression is abolished. Further, it was shown that visible and not ultraviolet or infrared light is responsible for the observed effects. The effect of light was an ocular event and was not developmentally mandated as is visual development, but inducible in adult dark-reared animals. These results showed that there is active intraocular T-cell regulation which prevents suppression in the absence of light. The purpose of this proposal is to further define the cellular and molecular events in the effect of visible light on intraocular immune reactions. The applicant would determine the wavelength responsible for inducing suppressed DTH in order to get an action spectrum that might reflect the nature of the molecular mediators in the effect. He would determine if altering the light/dark conditions changes the distribution and expression in the eye of class I, class II, and other molecules relevant to the immune response (CD4, Thy-1, LCA). He would analyze the regulatory cells induced following AC injection and removal of light from the eyes since these cells are capable of blocking the activity of Ts-cells. Further studies would determine the role of oxygen reactive compounds, neurotransmitters, and neuropeptides play in the light/dark regulation of intraocular immune reactions. The investigator believes that these studies have important implications for understanding intraocular immune responses and possibly the treatment of eye disease.