EFFECT OF LIGHT ON THE OCULAR IMMUNE RESPONSE

描述（改编自申请人摘要）：将抗原注射至 眼睛的前房（AC）导致独特的免疫模式， 前房相关免疫偏离（ACAID）， 其中抗原特异性抑制性T细胞（Ts细胞）抑制全身性 迟发型超敏反应（DTH）。 通过使用黑暗饲养和光明饲养 小鼠（Balb/c），先前证明可见光具有 直接影响眼内免疫反应。 如果光被阻止 从到达眼睛的黑暗饲养，通过放置轻饲养的动物， AC注射后的黑暗，或通过关闭光饲养的眼睑 动物在黑暗中AC注射后，或通过关闭眼睑 AC注射后光饲养动物的抑制被消除。 此外，本发明还 结果表明， 对观察到的效果负责。 光的影响是一个眼部事件， 强制性的视觉发展，但在成年黑暗饲养诱导 动物 这些结果表明，眼内存在活跃的T细胞， 在没有光的情况下防止抑制的调节。 目的 这一建议的目的是进一步定义细胞和分子事件， 可见光对眼内免疫反应的影响。 申请人 将决定负责诱导抑制DTH的波长， 为了得到一个行动谱，可能会反映的性质， 分子介体的作用。 他将决定是否改变 光/暗条件改变了眼睛的分布和表达， I类、II类和其他与免疫应答相关的分子 (CD4，Thy-1，LCA）。 他将分析以下诱导的调节细胞 AC注入和从眼睛中移除光，因为这些细胞是 能够阻断Ts细胞的活性。 进一步的研究将 确定氧反应化合物，神经递质的作用， 神经肽在眼内免疫的明/暗调节中的作用 反应. 研究人员认为，这些研究具有重要意义。 对理解眼内免疫反应的影响， 治疗眼疾。

DESCRIPTION (adapted from applicant's abstract): Injection antigen into the anterior chamber (AC) of the eye results in a unique pattern of immune responses known as Anterior Chamber Associated Immune Deviation (ACAID), in which antigen-specific suppressor T-cells (Ts-cells) inhibit systemic delayed type hypersensitivity (DTH). By using dark-reared and light-reared mice (Balb/c) it was previously demonstrated that visible light has a direct effect on this intraocular immune reaction. If light is prevented from reaching the eye by dark-rearing, by placing light reared animals in the dark post AC injection, or by closing the eyelid of light-reared animals in the dark post AC injection, or by closing the eyelid of light-reared animals post AC injection suppression is abolished. Further, it was shown that visible and not ultraviolet or infrared light is responsible for the observed effects. The effect of light was an ocular event and was not developmentally mandated as is visual development, but inducible in adult dark-reared animals. These results showed that there is active intraocular T-cell regulation which prevents suppression in the absence of light. The purpose of this proposal is to further define the cellular and molecular events in the effect of visible light on intraocular immune reactions. The applicant would determine the wavelength responsible for inducing suppressed DTH in order to get an action spectrum that might reflect the nature of the molecular mediators in the effect. He would determine if altering the light/dark conditions changes the distribution and expression in the eye of class I, class II, and other molecules relevant to the immune response (CD4, Thy-1, LCA). He would analyze the regulatory cells induced following AC injection and removal of light from the eyes since these cells are capable of blocking the activity of Ts-cells. Further studies would determine the role of oxygen reactive compounds, neurotransmitters, and neuropeptides play in the light/dark regulation of intraocular immune reactions. The investigator believes that these studies have important implications for understanding intraocular immune responses and possibly the treatment of eye disease.