CELLULAR CONTROL OF CYTOSKELETON ASSEMBLY

拟议研究的长期目标是确定 微管的组装和功能是如何通过 微管相关蛋白(MAP)。建议继续 我们对特定地图的分布和表达的研究， 图4，使用多种方法。地图4的位置，或 在小鼠早期胚胎或处于不同阶段的4个亚种 胎儿发育，将使用免疫细胞学进行分析 技巧。这些研究的结果将与 MAP-4基因表达的原位杂交分析 基因。第二组研究将采用显微注射和 光漂白后荧光重分布(FRAP) 研究MAP-微管相互作用如何的技术 在细胞中进行调制。建议继续研究 MAP上的动力学，使用修饰的蛋白质表位特异性 抗体，试图确定MAP分子的哪些区域 可能在体内微管的功能中起重要作用。一个 第三个项目涉及识别编码MAP 4的序列， 并使用这些克隆来量化MAP 4的表达， 或定位不同发育阶段的4个亚种。这些 研究应该提供有关生物作用的基本信息。 微管相关蛋白在组织中的作用 发育中的生物体和个体细胞中的微管。

The long term objective of the proposed research is to determine how microtubule assembly and function are modulated by microtubule-associated proteins (MAP). It is proposed to continue our studies on the distribution and expression of a particular MAP, MAP 4, using a number of approaches. The location of MAP 4, or MAP 4 subspecies, in early mouse embryos, or at various stages of fetal development, will be analyzed using immunocytological techniques. The results from these studies will be correlated with in situ hybridization analyses of the expression of the MAP 4 genes. A second set of studies will employ microinjection and fluorescence redistribution after photo-bleaching (FRAP) technologies to investigate how MAP-microtubule interactions are modulated in cells. It is proposed to continue studies on the dynamics on MAP, using modified proteins epitope specific antibodies, to try to ascertain which regions of the MAP molecule may be important in the functioning of microtubules in vivo. A third project involves identifying the sequences coding for MAP 4, and the use of these clones to quantify the expression of MAP 4, or MAP 4 subspecies during various developmental stages. These studies should provide basic information on the biological role of microtubule-associated proteins in the organization of microtubules in developing organisms and in individual cells.