GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST

其目的是了解真核基因的调控。 在核酸-蛋白质相互作用和代谢方面 效应分子的调节。具体目标是 确定受调控表达所需的DNA序列 并鉴定和表征与之相互作用的蛋白质 这些序列。二十二年中的特定核苷酸变化 碱基对二联体(上游激活位点，或UAS) 介导酵母乙醇脱氢酶2基因的调控 将被构造和表征以识别潜在的点 DNA和ADR1之间的接触，这种蛋白质可以 显然是在无人机上互动。ADR1的调节结构域 核进入，DNA结合，转录激活，以及 将通过对缺失突变的分析来确定调控。这个 可能的DNA结合和转录激活结构域 ADR1将通过经典遗传学和重组遗传学进行分析 (使用寡核苷酸定向突变)和通过构建 混合调控基因。推定的塔塔矿石的特征 由ADR6编码的CAP调节器系数将通过 遗传和生化手段。 更好地了解酒精新陈代谢和基因 可望控制乙醇的合成和降解 这些研究的医疗益处。基本了解如何 蛋白质控制基因的作用最终将有益于医学。 也是。

The objective is to understand the regulation of a eukaryotic gene in terms of nucleic acid-protein interaction and metabolic regulation of effector molecules. The specific aims are to characterize the DNA sequences required for regulated expression and to identify and characterize the proteins which interact with these sequences. Specific nucleotide changes in the twenty-two base pair dyad (the upstream activation site, or UAS) which mediates regulation of the alcohol dehydrogenase 2 gene of yeast will be constructed and characterized to identify potential points of contact between the DNA and ADR1, the protein which apparently interacts at the UAS. The domains of ADR1 mediating nuclear entry, DNA binding, transcriptional activation, and regulation will be identified by analysis of deletion mutants. The putative DNA binding and transcription activation domain of ADR1 will be analyzed by classical and recombinant genetics (using oligonucleotide directed mutagenesis) and by constructing hybrid regulatory genes. Characterization of a putative TATA or CAP regulator factor encoded by ADR6 will be achieved by genetic and biochemical means. A better understanding of alcohol metabolism and the genes controlling ethanol synthesis and degradation are anticipated medical benefits of these studies. A basic understanding of how proteins control gene action will ultimately be of medical benefit as well.