CHARACTERIZATION OF BASEMENT MEMBRANE GLYCOPROTEINS

本提案的长期目标是了解组织 基底膜及其在正常和病理状态中的作用。 基底膜是一种复杂的细胞外基质， 组织组织、胚胎发生、细胞粘附、过滤和 保护 在许多情况下，基底膜结构发生改变， 病理状态如糖尿病、癌症、大疱性类天疱疮， 哮喘、各种来源的肾小球肾炎和几种自身免疫性疾病， 疾病 这项建议的具体目标有两个方面： 基底膜组织将研究与模型矩阵产生 和（B）基因的调节 对于层粘连蛋白和巢蛋白， 探讨了 将采用的技术包括构建 杂交瘤细胞产生检测基底膜的特异性探针 组织，肽分离和测序，分离和 表征编码层粘连蛋白基因的DNA序列， 巢蛋白和用维甲酸诱导胚胎癌F9细胞 和二丁酰环磷酸腺苷（dibutyryl cyclicAMP）来研究层粘连蛋白和巢蛋白的表达。

The long term objective of this proposal is to understand the organization of basement membranes and their role in normal and pathological states. Basement membranes are complex extracellular matrices that are involved in tissue organization, embryogenesis, cell adhesion, filtration and protection. There are alterations in basement membrane structures in many pathological states such as diabetes mellitus, cancer, bullous pemphigoid, asthma, glomeruluo-nephritis of varied origin and several of the autoimmune diseases. The specific objectives of this proposal are two fold: (a) basement membrane organization will be studied with a model matrix produced in cell culture by mouse endoderm cells and (b) the regulation of the genes for laminin and entactin, two basement membrane components will be explored. The techniques that will be employed include the construction of hybridoma cells to generate specific probes for examining basement membrane organization, peptide isolation and sequencing, isolation and characterization of DNA sequences that code for the genes of laminin and entactin, and induction of embryonal carcinoma F9 cells with retinoic acid and dibutyryl cyclic AMP to study the expression of laminin and entactin.