BIOCHEMISTRY OF GENE EXPRESSION IN HIGHER EUKARYOTES

这项研究计划的三个主要目标中的两个 格兰特与最初的提议保持不变。其中第一个涉及到 使用生物化学和遗传方法的组合来破译 组蛋白乙酰化在高等真核生物中的生物学作用。第二 我们的目标是揭示这一过程的一些分子细节 在胚胎发育过程中，位置信息被传递给细胞，用作 模型系统以“前后位区域”为标准 雏鸡早期肢芽的“极化活动”(ZPA)。一个新的目标 关于确定哺乳动物II型DNA的生物学作用的问题 拓扑异构酶，我们最近在实验室发现的新酶 嫌疑人与真核细胞DNA复制的启动密切相关。

Two of the three major objectives of the research program supported by this grant remain unchanged from the original proposal. The first of these involves the use of a combination of biochemical and genetic approaches to decipher the biological role of histone acetylation in higher eukaryotes. The second objective is to uncover some of the molecular details of the process whereby positional information is imparted to cells during embryogenesis, using as a model system the specification of anterio-posterior position by the "zone of polarizing activity" (ZPA) in the early chick limb bud. A newer objective concerns determining the biological role of mammalian type II DNA topoisomerases, new enzymes recently discovered in this laboratory which we suspect are intimately involved in the initiation of eukaryotic DNA replication.