Visualising chromatin changes in 3 dimensions: super to ultra resolution

三维可视化染色质变化：超分辨率到超分辨率

• 批准号: DP230103211
• 负责人: Prof Stephen Turner
• 金额: $ 41.2万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP230103211
• 关键词: Visualising; chromatin; changes; dimensions; super

Packaging of genomic information into the nucleus of a cell necessitates the formation of tightly compacted and highly organized genomic structures within the nucleus, a configuration that is inherently repressive for gene transcription. Hence, mechanisms that alter the spatial organisation of DNA are critical to enable a variety of genome functions, including DNA transcription. This proposal will utilise novel adaptations of super resolution microscopy to visualise in 3 dimensions how changes in chromatin modifications impact genome spatial organisation within the nucleus, and how this then links to cellular differentiation. This will provide a picture of how spatial organisation within the nucleus supports general cell differentiation.

将基因组信息包装到细胞核中需要在细胞核内形成紧密压缩和高度组织化的基因组结构，这种结构对基因转录具有固有的抑制作用。因此，改变DNA空间组织的机制对于实现包括DNA转录在内的各种基因组功能至关重要。该提案将利用超分辨率显微镜的新适应性，以三维方式可视化染色质修饰的变化如何影响细胞核内的基因组空间组织，以及这如何与细胞分化联系起来。这将提供一幅细胞核内空间组织如何支持一般细胞分化的图片。