DEVELOPMENTAL GENETICS OF CAENORHABDITIS ELEGANS

拟议研究的总体目标是扩大遗传分析的范围 自由生活线虫秀丽线虫的发育 研究动物发育和遗传基础的模式生物 行为，因为它的大小和细胞数量有限而被选择，它的僵化 特定的发育及其对基因操作的适用性。一个 理解发育的遗传基础可能是非常重要的。 并可能最终为我们提供重要信息 许多问题，从先天缺陷到衰老。更多 具体目标是：第一，继续进行基因分析 由游离染色体片段的体细胞有序丢失所产生的马赛克 定位已知影响的各种基因作用的解剖焦点 动物的行为、发育或解剖学；第二，识别和 描述某些细胞为XX(正常情况下)的雌雄同型 雌雄同体)和其他细胞是XO(通常为雄性)；第三，为了识别 并描述了新的染色体复制，包括自由和易位 以及X复制和常染色体复制，这两种复制都将使用 平衡隐性致死突变和不育突变并产生基因 马赛克；第四，标记几个影响神经系统的基因 利用转座子Tc1进行克隆并利用Tc1标签进行克隆 至少这些基因中的一个；第五，继续制造，在 除了染色体复制，重新排列的染色体可以起到 显性交叉抑制器，可用于平衡隐性 致命的和无菌的突变。

The overall goal of the proposed research is to extend the genetic analysis of the development of the free-living nematode Caenorhabditis elegans, a model organism for studying the genetic basis of animal development and behavior, chosen because of its limited size and cell number, its rigidly specified development, and its suitability for genetic manipulation. An understanding of the genetic basis of development may well be fundamental to much of medicine and may ultimately contribute important information to many problems, ranging from congenital defects to senescence. More specific objectives are: first, to continue the analysis of genetic mosaics produced by the somatic loss of free chromosome fragments in order to localize the anatomical foci of action of various genes known to affect animal behavior, development or anatomy; second, to identify and characterize gynandromorphs in which some cells are XX (normally hermaphrodite) and other cells are XO (normally male); third, to identify and characterize new chromosome duplications, both free and translocated and both X duplications and autosomal duplications, which will be used both to balance recessive lethal and sterile mutations and to generate genetic mosaics; fourth, to tag several genes that affect nervous system development with the transposon Tc1 and to make use of the Tc1 tag to clone at least one of these genes; and fifth, to continue manufacturing, in addition to chromosome duplications, rearranged chromosomes that can act as dominant crossover suppressors, which can be used to balance recessive lethal and sterile mutations.