LEUKOCYTE FUNCTION IN THE PATHOLOGY OF BURN INJURY

这项研究旨在阐明 可识别的生化畸变导致异常 多形核白细胞功能，伤口事件，和全身 在热损伤患者中观察到的变化。 这些研究将侧重于： 1)确定氧化功能降低的机制和状态， 完整白细胞中的活性氧; 2）将这些 对烧伤血清/血浆或体液中因子的氧化反应; 3） 分离和表征影响的血清和流体因子 氧化过程，从而杀死细菌; 4）将 具有结构变化的显性功能性O2异常， 电子显微镜和组织化学测定来表征 循环和伤口流体细胞群;和5）试图将 伤口环境（细胞和液体）观察到的类型 细菌在烧伤伤口上繁殖 对生化事件的理解 在中性粒细胞功能及其与细菌定植的关系中是至关重要的 在适当的局部或全身治疗干预之前， 考虑。

The proposed research is designed to clarify the relationship between identifiable biochemical aberrations responsible for abnormal polymorphonuclear leukocyte function, the wound events, and systemic changes seen in thermally injured patients. The studies will be focused: 1) to identify mechanisms of decreased oxidative functions and the status of reactive oxygen species in intact leukocytes; 2) to relate these oxidative responses to factors in burn serum/plasma or would fluid; 3) to isolate and characterize the sera and fluid factor(s) which affect oxidative processes and therefore bacterial kill; 4) to correlate the domonstrated functional O2 abnormalities with structural changes by electron microscopy and histochemical determinations to characterize circulating and wound fluid cell populations; and 5) to attempt to relate wound environment (cellular and fluid) observations to the types of organisms colonizing burn wounds. Understanding of the biochemical events in PMN function and their relation to bacterial colonization is essential before appropriate local or systemic therapeutic interventions can be contemplated.