Optical Fluorescence Micro and Nanoscopy to determine and quantify functional molecular interactions and dynamics across time and length scales

光学显微镜是现代生物科学和医学研究的基石，它允许从单个分子到生物体的各个尺度上对过程进行检查。在这里，我们建议购买一个最近发布的高端系统，使我们能够执行最先进的“超分辨率”显微镜，并有可能在分子水平上执行时间分辨成像。这将使我们能够以无与伦比的空间和时间分辨率研究细胞中的事件。至关重要的是，这些系统将允许bbsrc资助的研究人员和其他研究人员推动研究项目，了解分子驱动的基本机制，其相互作用和动力学，从单个细胞水平到细胞的集体功能组装。该显微镜将成为帝国理工学院“成像和光学显微镜设施”（FILM）的一部分，并向学院的所有成员以及当地，国家和国际合作者开放。

Light microscopy forms a bedrock of modern research in bioscience and medicine, allowing processes to be examined across scales from individual molecules up to living organisms. Here we propose to acquire a high-end recently released system to enable us to perform state-of-the-art "super resolution" microscopy, with the possibility to perform time-resolved imaging at molecular level. This will allow us to study events in cells with unparalleled spatial and temporal resolution. Critically, these systems will permit BBSRC-funded and other researchers to drive research projects understanding the fundamental mechanisms driven by molecules, its interactions and dynamics at the single cellular level up to collective functional assemblies of cells. The microscope will be part of Imperial's "Facility for Imaging and Light microscopy" (FILM) and accessible to all members of College, as well as local, national and international collaborators.