A hyperspectral reflectance and fluorescence micro-endoscopic system for diagnostics and therapy

用于诊断和治疗的高光谱反射和荧光显微内窥镜系统

• 批准号: 418689-2012
• 负责人: Douplik, Alexandre
• 金额: $ 1.46万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=573791
• 关键词: hyperspectral; reflectance; fluorescence; micro; endoscopic

The focus of the study is a spectral imaging detection of cancer or other pathological lesions in hardly approachable ducts such as breast milk, pancreatic, Biliary ducts. The malignancies in breasts, Biliary ducts, gold bladder, pancreas are frequently developed within the duct epithelium, and hence detectable via ducts. Most of these malignancies are aggressive leaving a short time (months) for in-time diagnostics and successful consequent surgical interventions. The primary aim of the project is to develop and test a versatile research micro-endoscopic system. The work objectives include development of a tunable spectral band endoscopic light source based on acousto-optical filter for spectral reflectance imaging and laser diode array for excitation-emission imaging data collection to facilitate imaging of tissue morphological parameters, cell metabolism and local blood content. The biotissue examination by mean of the optical technology proposed will be combined with traditional diagnostic technique such as pathohistology for validation and improvement. As a result of the study, a high-qualified personal will be trained in the field of Biophotonics in academic-clinical-industrial environment. It is anticipated that the project will contribute into the development of a strategical direction bringing better understanding of the basic mechanisms of pathological development in ducts, microscale high resolution imaging technique, new generation micro-endoscopy technology including endoscopic light therapy with simultaneous guidance and monitoring, development of realistic tissue phantoms to reduce usage of lab animals and may lead to the development of the micro-endoscopic video facility and super resolution (subwavelength domain) endoscopic modalities.

该研究的重点是在难以接近的管道（如母乳、胰腺、胆管）中的癌症或其他病理病变的光谱成像检测。乳腺、胆管、金膀胱、胰腺的恶性肿瘤通常发生在导管上皮内，因此可通过导管检测。这些恶性肿瘤中的大多数是侵袭性的，留下很短的时间（数月）用于及时诊断和成功的后续手术干预。该项目的主要目的是开发和测试一个多功能的研究显微内窥镜系统。工作目标包括开发基于声光滤波器的可调谐光谱带内窥镜光源，用于光谱反射成像和激光二极管阵列，用于激发发射成像数据收集，以促进组织形态学参数，细胞代谢和局部血液含量的成像。利用光学技术进行的生物组织检查将与病理组织学等传统诊断技术相结合，以进行验证和改进。作为研究的结果，高素质的个人将在学术-临床-工业环境中接受生物光子学领域的培训。预计该项目将有助于发展一个战略方向，更好地了解导管病理发展的基本机制，微尺度高分辨率成像技术，新一代显微内窥镜技术，包括同时引导和监测的内窥镜光疗，开发逼真的组织模型，以减少实验室动物的使用，并可能导致微内窥镜视频设施和超分辨率（亚波长域）内窥镜模式的发展。