X-RAY CRYSTALLOGRAPHIC ANALYSES OF PROTEIN STRUCTURES

这项研究将建立精确定义的蛋白质结构系统 通过应用X射线结晶学技术来达到以下目的 为生化实验提供了良好的物理基础。分析 将对几种蛋白质进行高分辨率的研究，最初将 包括肌酸激酶、abrin、过氧化氢酶、铁蛋白、刀豆蛋白、刀豆蛋白B 和α-淀粉酶。分离，结晶，一些生化反应 表征和初步的X射线衍射研究已经 对其中的每一个都进行了研究。 重点将放在那些最具生物学意义的蛋白质上。 (肌酸激酶、abrin、α-淀粉酶、过氧化氢酶和铁蛋白)与 目的通过对它们的研究来阐明它们的机制特征 结构在其各自底物、辅酶存在时的转变 通过使用差分傅立叶技术来描述类似物和效应器。在……里面 此外，这些研究将检查独特的结构特征，如 糖蛋白、病毒模型和几个独特的四元结构。 将开发一种提取低分辨率相位信息的方法 用于加速X射线结构分析的电子显微照片。 此外，还将建立一个系统，用于数字和光学过滤 电子显微镜照片。

This research will establish precisely defined protein structural systems through the application of X-ray crystallographic techniques for the purpose of providing a sound physical basis for biochemical experimentation. The analysis of several proteins to high resolution will be undertaken which initially will include creatine kinase, abrin, catalase, ferritin, canavalin, concanavalin B, and alpha-amylase. Isolation, crystallization, some biochemical characterization and preliminary X-ray diffraction investigations have already been conducted on each of these. Emphasis will be placed on those proteins of most relevant biological interest (creatine kinase, abrin, alpha-amylase, catalase and ferritin) with the objective of elucidating their mechanistic features by examining their structures in the presence of their respective substrates, coenzymes, transition state analogues and effectors by employing the difference Fourier technique. In addition, the studies will examine unique structural features such as a glycoprotein, a virus model, and several unique quaternary structures. A method will be developed for extracting low resolution phase information from electron micrographs for the purpose of expediting X-ray structural analyses. In addition, a system will be set up for digital and optical filtering of electron micrographs.