DYNAMICS OF ACTIN IN NORMAL AND TRANSFORMED CELLS

我们研究的目的是了解它的动态特性 肌动蛋白和肌球蛋白在活细胞中的表达及其机制 收缩结构的装配和拆卸。在现在 项目中，我们将重点研究肌动蛋白和肌球蛋白的运动 活的成纤维细胞中的细丝，荧光标记的检查 具有微光视频的外源或内源细丝 显微镜和数字图像处理。我们将首先进行微量注射 肌动蛋白细丝片段，用荧光鬼笔花素标记。 他们的行动将通过直接跟踪 位置和荧光恢复后，光漂白。 类似的研究将在荧光标记的 内源性肌球蛋白细丝和聚苯乙烯荧光小球 包裹着肌球蛋白。这些结构的运动将是 在细胞的不同区域进行分析，并将进行关联 伴随着细胞运动。此外，转型的影响 在这些细丝上模拟变形的处理将是 检查过了。我们将进一步研究肌动蛋白细丝的混合 细胞融合后，细丝运动的作用和 封闭在各种收缩结构的形成中。 这些结果不仅有助于我们理解正常的功能 含有肌动蛋白的结构，但也包括由 致癌转化。

The goal of our research is to understand the dynamic properties of actin and myosin in living cells and the mechanisms of the assembly and disassembly of contractile structures. In the present project, we will focus on the movement of actin and myosin filaments in living fibroblasts, ty examining fluorescently labeled exogenous or endogenous filaments with low light level video microscopy and digital image processing. We will first microinject fragments of actin filaments labeled with fluorescent phalloidin. Their movements will be studied both by directly tracking the position and by fluorescence recovery after photobleaching. Similar studies will be performed on fluorescently labeled endogenous myosin filaments, and on fluorescent polystyrene beads coated with myosin. The movement of these structures will be analyzed in different regions of the cell and will be correlated with cell locomotion. In addition, the effects of transformation and treatments mimicking transformation on these filaments will be examined. We will further study the intermixing of actin filaments after cell fusion, and the role of filament movement and sequestration in the formation of various contractile structures. These results will help us understand not only the normal functions of actin-containing structures, but also the disruptions caused by oncogenic transformation.