E- AND 1H TRANSLOCATION IN ENERGY CONVERSION

本研究项目的目的是调查 分子内和分子间结构的耦合 生物体内电子和质子转移到ATP合成 膜。 通过确定的整体形状， 中等分辨率下的相关膜分子组分 以及它们的位置（包括它们的氧化还原的位置 中心）相对于彼此以及相对于整个膜 结构，预计将获得实质性的见解 关于电子和质子的可能机制 转运和能量耦合。 技术非- 共振和共振X射线与中子衍射 （扩展X射线吸收精细结构）光谱学，光学 线性二向色性，三维电子显微图像 FRAP（Fluorescence Recovery After） 光漂白）将被用来研究相关的膜 包括光合反应中心、细胞色素 氧化酶、细胞色素c和细胞色素B/c1以及各种 其复合物主要以单一的、定向的 单层膜系统，并且在某些情况下， 膜多层系统。 反应中心-细胞色素 B/c1-细胞色素c复合物表现出高效的光驱动 电子转移反应的循环系列。

The purpose of this research project is to investigate the role of intramolecular and intermolecular structure in the coupling of electron and proton translocation to ATP synthesis in biological membranes. Through a determination of the overall shapes of relevant membrane molecular components at moderate resolution and of their positions (including the positions of their redox centers) relative to each other and to the overall membrane structure, it is expected that substantial insight will be gained concerning the possible mechanisms of electron and proton translocation and of energy coupling. The techniques of non- resonance and resonance X-ray and neutron diffraction, EXAFS (Extended X-ray Absorption Fine Structure) spectroscopy, optical linear dichroism, three-dimensional electron microscopic image reconstruction, and FRAP (Fluorescence Recovery After Photobleaching) will be utilized to study the relevant membrane proteins including photosynthetic reaction centers, cytochrome oxidase, cytochrome c and cytochrome b/c1 and various complexes thereof primarily in the form of single, oriented monolayer membrane systems and in some instances oriented membrane multilayer systems. The reaction center-cytochrome b/c1-cytochrome c complexes exhibit an efficient light-driven cyclic series of electron transfer reactions.