A WEIN ANALYZER FOR HIGH MASS SEPARATION OF BIOORGANICS

在前两年的努力中，我们已经证明了Wien 质谱仪(WMS)能够分析极高质量的化合物 ((CsI)~(155)Cs~+在40,433天)，并且具有非常高的灵敏度(1 pmol牛 胰岛素治疗5,734d)。每个样品大约10分钟的短分析时间允许 快速优化仪器操作条件，如样品 基质组成和基质温度。我们的主要目标将是 建立LSIMS技术的高质量和高灵敏度能力 并与加州大学旧金山分校和其他地方的研究人员合作 生化工程和结构中的各种问题 生物化学。为了支持这些目标，在 建议使用WMS： 1)安装功能更强大的Wien分析仪，可增加质量 分辨率提高到原来的四倍，而不降低灵敏度。这将是 将质量测量的准确度提高相称的量； 2)提高抽油速度，降低仪器背景压力。这 将提高高质量化合物的透射率和分辨率； 3)为负面分析提供工具能力 带电化合物； 4)继续改进离子源、探测器和探测器，以增加 仪器的灵敏度。

During the previous two years of effort, we have demonstrated that the Wien mass spectrometer (WMS) is capable of analyzing compounds of very high mass ((CsI)155Cs+ at 40,433d) and with very high sensitivity (1 pmol of Bovine insulin at 5,734d). The short analysis time of about 10 min/sample allows rapid optimization of instrumental operating conditions such as sample matrix composition and matrix temperature. Our primary goals will be to establish the upper mass and sensitivity capability of the LSIMS technique on the WMS and to collaborate with researchers at UCSF and elsewhere on a wide variety of problems in biochemical engineering and structural biochemistry. In support of these goals, several major improvements in the WMS are proposed: 1) Install a more powerful Wien analyzer capable of increasing mass resolution by a factor of four, without reducing sensitivity. This will increase the accuracy of mass measurement by a commensurate amount; 2) Reduce instrument background pressure by increasing pumping speed. This will improve transmission and resolution for high mass compounds; 3) Provide the instrumental capability for the analysis of negatively charged compounds; 4) Continue refinements on the ion source, probe and detector to increase sensitivity of the instrument.