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NMR STUDIES OF METABOLIC PATHWAYS IN VIVO AND IN VITRO

NMR STUDIES OF METABOLIC PATHWAYS IN VIVO AND IN VITRO
体内和体外代谢途径的 NMR 研究
批准号：
3281596
负责人：
ALASTAIR I SCOTT
金额：
$20.48万
依托单位：
TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
项目状态：
已结题
起止时间：
1983-03-01 至 1988-02-29
来源：
https://reporter.nih.gov/project-details/3281596
关键词：
Fungi Krebs' cycle bacteria biotransformation cell free system cephalosporins chemical reaction chemical synthesis cholesterol chymotrypsin clinical biomedical equipment cryoscience eicosanoid metabolism erythrocytes fatty acid biosynthesis fatty acid synthase fermentation fibroblasts gluconeogenesis glycolysis human tissue luciferin monooxygenase metalloenzyme methionine methylmalonyl coA epimerase neoplastic cell noninvasive diagnosis nuclear magnetic resonance spectroscopy nutrition related tag o aminobenzoate pancreatic ribonuclease papain penicillins plants prostaglandin endoperoxide synthase purine /pyrimidine metabolism radiopharmacology squalene stable isotope diagnosis steroid biosynthesis tetracyclines tissue /cell culture trypsin vitamin B12 compound vitamin biosynthesis

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中文摘要
翻译
发展使用宽口径、高场（300-500兆赫）、 高分辨率核磁共振波谱学研究生命代谢过程 系统和体外将进行。 使用Multiple Labeled 通过区域特异性合成制备的底物（13 C，15 N，2 H）将是 在分配化学位移未知和短命物种的价值。 它 建议使用低温酶学和流动池来研究 生物化学反应中的碳、氢和氮， 迄今未被发现的物种，并制定非侵入性的方法， 分析微克数量的生物学重要分子， 在哺乳动物、细菌和植物细胞中的代谢，以及整体， 灌注的器官和昆虫。
英文摘要
Development of techniques using wide-bore, high-field (300-500 MHz), high-resolution NMR spectroscopy to study metabolic processes in living systems and in vitro will be undertaken. The use of multiply labeled substrates (13C, 15N, 2H) prepared by regiospecific synthesis will be of value in assigning chemical shifts to unknown and short-lived species. It is proposed to use cryoenzymology and flow cells to study the flux of carbon, hydrogen and nitrogen in biochemical reactions, to identify hitherto undetected species and to devise non-invasive methods for the analysis of microgram quantities of biologically important molecules during metabolism in mammalian, bacterial and plant cells, as well as whole, perfused organs and insects.
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