A WIEN ANALYZER FOR HIGH MASS SEPARATION OF BIOORGANICS

在过去的几年里，新的电离技术的发展已经 使质谱分析技术得以扩展到令人兴奋的新领域 研究，如蛋白质测序和结构分析， 大的化学和热不稳定的生物分子。 似乎 然而，目前的质量分析器存在局限性， 可能包括低透射率、有限的质量范围和低分辨率。 Wien质谱仪，其操作能力高， 光束能量具有高透射率， 大约10，000的分辨率应该为 质量分析可用的方法。 我们最初的目标是 在前人设计的基础上，构建了一台维恩滤波质谱仪 经验，将具有以下能力：1）质量分辨率 2）大于100，000的质量范围（Wien过滤器基本上 无质量上限）; 3）加速电压范围为4，000至50，000 4）位置敏感电光耦合离子检测器， 允许100%占空比的样品光谱的一部分的积分 周期 该仪器最初将与液体西姆斯和现场 解吸类型的离子源，以从高质量 （3，000 - 100，000道尔顿）生物有机样品。 质量秤的校准 将使用碱金属卤化物盐离子簇作为质量建立 标记。 经过初步测试和评估的质量 光谱仪，该仪器将被应用到重要的问题， 各种高质量有机样品的结构解析， 生物聚合物、蛋白质、激素等。最后，我们将评估 Wien分析仪的性能，以确定其整体有效性， 高质量的分析仪器，并提出进一步完善其 设计

Over the past few years, the development of new ionization techniques has permitted the expansion of mass spectrometry into new and exciting fields of research such as protein sequencing and structural analysis of very large chemically and thermally labile biological molecules. It appears now, however, that there are limitations in present mass analyzers which can consist of low transmission, restricted mass range and low resolution. The Wien mass spectrometer, with its capabilities of operating with high beam energy with high transmission and virtually no upper mass limit at a resolution of about 10,000 should offer a significant addition to the available methodology for mass analysis. Our initial object is to construct a Wien filter mass spectrometer, based on previous design experience, that will have the following capabilities: 1) mass resolution of 10,000; 2) mass range greater than 100,000 (the Wien filter essentially has no upper mass limit); 3) acceleration voltage range of 4,000 to 50,00 V; and 4) position sensitive electrooptically coupled ion detector to permit integration of a portion of the sample spectrum with 100% duty cycle. The instrument initially will be used with liquid SIMS and field desorption types of ion sources to produce ions from high mass (3,000-100,000 daltons) bioorganic samples. Calibration of the mass scale will be established using alkali metal halide salt ion clusters as mass markers. After preliminary testing and evaluation of the mass spectrometer, the instrument will then be applied to important problems in structural elucidation of a variety of high mass organic samples such as biopolymers, proteins, hormones, etc. Finally, we will evaluate the performance of the Wien analyzer to determine its overall effectiveness as a high mass analytical instrument and to propose further refinements in its design.