A new platform for multiplexed longterm imaging of cell behaviour during regeneration and development.

能够使用显微镜为细胞成像是许多生物研究项目的核心。跟踪活细胞以了解它们的行为通常是有用的。研究工具，如荧光蛋白，可以让人们实时研究细胞的微小细节。然而，致密组织中的细胞成像尤其困难，因为上面和下面的细胞会干扰图像捕获。为了部分解决这一问题，已经开发出了减少干扰的显微镜，这种显微镜可以从侧面用“光片”照射样品。需要进一步的技术来跟踪长时间的细胞，如几天。为了长期保持健康，组织有特殊的环境需求，而且在样本上使用太多的光线也可能是危险的。此外，如果细胞移动，我们需要显微镜自动跟踪它们。我们的研究人员要求购买一种先进的显微镜，克服这些障碍，使致密组织能够成功地进行长期成像。

Being able to image cells using microscopy is central to many biological research projects. It is often useful to follow living cells to understand their behaviour. Research tools such as fluorescent proteins are available that let one study the minute details of cells in realtime. However, imaging cells within a dense tissue is particularly difficult as the cells above and below interfere with image capture. To partially overcome this issue microscopes that illuminate samples from the side with a "light sheet" have been developed that reduce interference. Further technology is needed to track cells over long periods such as days. Tissues have special environmental needs in order to stay healthy for long periods, and using too much light on the sample can also be hazardous. In addition, if the cells move we need the microscope to follow them automatically. Our researchers are requesting the purchase of an advanced microscope that overcomes these hurdles to enable successful longterm imaging of dense tissues.