NMR STUDIES OF THE MECHANISM OF ACTION OF TRP-REPRESSOR-

长期目标是在分子水平上了解 阻遏物识别DNA的特定序列， 代谢物对这种识别过程的调节。 具体目标 是研究热力学和机制的相互作用， trp-阻遏物及其操纵基因DNA和其他非操纵基因DNA， 特异性调节分子（色氨酸， 脱氨基色氨酸）。 阻遏蛋白和DNA也将被 在结构和功能上都有广泛的特点， 先进的高分辨率核磁共振方法，特别是二维方法 技术和测量弛豫时间。 一旦组件 被充分表征，在形成各种变化时发生的变化 复合物将进行比较，并详细介绍有关分子 将提供这种认可过程的依据。 这些识别 过程是基因表达控制的一个组成部分， 它是现代分子生物学的核心， 抑制某些癌症的发展。

The long term objectives are to understand at the molecular level the recognition of specific sequences of DNA by repressors, and the allosteric modulation of this recognition process by metabolites. The specific aims are to investigate the thermodynamics and mechanism of the interaction of trp-repressor with its operator DNA and other, non-operator DNA, in the presence and absence of specific modulatory molecules (tryptophan, desaminotryptophan). The repressor protein and the DNA will also be extensively characterized, both structurally and functionally, using advanced high resolution NMR methods, particularly two dimensional techniques and measurements of relaxation times. Once the components have been fully characterized, the changes that occur on forming the various complexes will be compared and detailed information regarding the molecular basis for such recognition processes will be available. These recognition processes are an integral part of the control of gene expression, a topic which lies at the heart of modern molecular biology, and relates indirectly to the development of suppression of certain kinds of cancer.