NMR STUDIES OF THE MECHANISM OF ACTION OF TRP-REPRESSOR-

长期目标是在分子水平上理解 抑制物对DNA特定序列的识别，以及变构 代谢物对这种识别过程的调节。具体目标 是为了研究分子间相互作用的热力学和机理 色氨酸阻遏物及其操纵子DNA和其他非操纵子DNA在 存在和不存在特定的调节分子(色氨酸， 去氨基色氨酸)。阻遏蛋白和DNA也将 在结构和功能上都具有广泛的特点，使用 先进的高分辨率核磁共振方法，特别是二维 松弛时间的技术和测量。一旦组件具有 已经完全刻画了，在形成各种变化上发生的 将对络合物进行比较，并提供关于分子 将提供此类认可过程的基础。这些认可 过程是基因表达控制不可或缺的一部分，这是一个主题 它位于现代分子生物学的核心，并间接地与 对某些类型癌症的抑制作用的发展。

The long term objectives are to understand at the molecular level the recognition of specific sequences of DNA by repressors, and the allosteric modulation of this recognition process by metabolites. The specific aims are to investigate the thermodynamics and mechanism of the interaction of trp-repressor with its operator DNA and other, non-operator DNA, in the presence and absence of specific modulatory molecules (tryptophan, desaminotryptophan). The repressor protein and the DNA will also be extensively characterized, both structurally and functionally, using advanced high resolution NMR methods, particularly two dimensional techniques and measurements of relaxation times. Once the components have been fully characterized, the changes that occur on forming the various complexes will be compared and detailed information regarding the molecular basis for such recognition processes will be available. These recognition processes are an integral part of the control of gene expression, a topic which lies at the heart of modern molecular biology, and relates indirectly to the development of suppression of certain kinds of cancer.