MOLECULAR GENETICS AND CELL BIOLOGY OF THE YEAST HEAT SH

真核细胞和原核细胞对温和的温度升高都有反应 (和许多其他形式的压力)通过诱导一小群人的合成 一种叫做热休克蛋白(HSPs)的高度保守的蛋白质。物美价廉 大量间接证据表明，这些蛋白质提供保护 热的毒性作用，但对它们的具体情况知之甚少 分子功能。最近的研究表明，其中一些蛋白质是 在正常发育过程中诱导的，这表明它们也可能 在正常的细胞过程中起着重要作用。我们希望调查一下 酵母的热休克反应，酿酒酵母，因为 这种生物为基因操作提供了许多独特的优势。 我们正在制造几种酵母菌的突变 热休克基因并产生针对这些蛋白质的抗体。这些 突变体和抗体将被用作研究其功能和 蛋白质在正常生长、产孢量和暴露过程中的调节 来施加压力。

Both eukaryotic and prokaryotic cells respond to mild temperature elevation (and many other forms of stress) by inducing the synthesis of a small group of highly conserved proteins called the heat-shock proteins (HSPs). A good deal of indirect evidence suggests that these proteins provide protection from the toxic effects of heat, but little is known about their specific molecular functions. Recent work has shown that some of these proteins are induced during the normal course of development, suggesting they may also play important roles in normal cellular processes. We wish to investigate the heat-shock response of the yeast, Saccharomyces cerevisiae, because this organism offers so many unique advantages for genetic manipulation. We are in the process of creating mutations in several of the yeast heat-shock genes and producing antibodies specific for the proteins. These mutants and antibodies will be used as tools to study the function and regulation of the proteins during normal growth, sporulation and exposure to stress.