MOLECULAR BIOLOGY OF MITOSIS IN ASPERGILLUS

对有丝分裂及其调控和机制的理解是 医学的许多领域都与细胞生长和分裂有关，例如， 癌症、胚胎发育和人类遗传学。的目标是 这个项目是对机械和规则的更好的理解 核子部门。直接的目标是识别和描述 利用DNA技术获得参与核分裂的特定基因产物 补体特异性有丝分裂突变的介导性转化 尼杜拉曲霉。基因将被克隆、测序和它们的产品 具有混合选择和互补翻译的特点 这些基因的蛋白产物的抗体将是 制备并用于在细胞中定位基因产物。

An understanding of mitosis, its regulation and its mechanics is central to many areas of medicine having to do with cell growth and division, e.g., cancer, embryological development and human genetics. The objective of this project is a better understanding of the mechanics and regulation of nuclear division. The immediate goal is to identify and characterize the products of specific genes involved in nuclear division by using DNA mediated transformation to complement specific mitotic mutations in Aspergillus nidulans. Genes will be cloned, sequenced and their products characterized by hybrid selection and translation of complementary messenger RNA's. Antibodies to the protein products of these genes will be prepared and used to localize the gene products in cells.