MOLECULAR GENETICS AND CELL BIOLOGY OF THE YEAST HEAT SH

真核细胞和原核细胞对温和的温度升高都有反应 (and许多其他形式的压力）通过诱导一个小团体的合成 一种叫做热休克蛋白（HSPs）的高度保守蛋白质。 一个好 大量间接证据表明，这些蛋白质提供保护， 从热的毒性作用，但很少有人知道他们的具体 分子功能 最近的研究表明，其中一些蛋白质是 在正常发育过程中诱导，这表明它们也可能 在正常的细胞过程中发挥重要作用。 我们希望调查 热休克反应的酵母，酿酒酵母，因为 这种生物体为基因操作提供了许多独特的优势。 我们正在对几种酵母进行突变 热休克基因并产生针对这些蛋白质的抗体。 这些 突变体和抗体将被用作研究功能的工具， 在正常生长、孢子形成和暴露期间调节蛋白质 压力

Both eukaryotic and prokaryotic cells respond to mild temperature elevation (and many other forms of stress) by inducing the synthesis of a small group of highly conserved proteins called the heat-shock proteins (HSPs). A good deal of indirect evidence suggests that these proteins provide protection from the toxic effects of heat, but little is known about their specific molecular functions. Recent work has shown that some of these proteins are induced during the normal course of development, suggesting they may also play important roles in normal cellular processes. We wish to investigate the heat-shock response of the yeast, Saccharomyces cerevisiae, because this organism offers so many unique advantages for genetic manipulation. We are in the process of creating mutations in several of the yeast heat-shock genes and producing antibodies specific for the proteins. These mutants and antibodies will be used as tools to study the function and regulation of the proteins during normal growth, sporulation and exposure to stress.