ATRESIA IN THE CYCLING RAT

这个项目的总体目标是确定毛囊的原因。 骑自行车的大鼠出现闭锁。抗睾酮抗血清将被注射到 检验这样的假设：雄激素在卵泡内的积累是 闭锁的直接原因。以确定黄体生成素激增是否促进闭锁，以及 事先暴露于卵泡刺激素可防止围排卵期的成分--闭锁 大量的促性腺激素会被解离。猪卵泡液 (“抑制素”)将被用来减弱FSH激增；外源性FSH和黄体生成素将 用来“促进”或增强内源性促性腺激素激增。这个 不同处理方法对小有腔卵泡数量和存活率的影响 (那些特别容易闭锁的卵泡)，将由 组织学方法。将为以下顺序建立时间进程 与这些卵泡的闭锁有关的形态学事件。小鼻窦 卵泡将被分离并放置在组织培养中，以确定是否暴露于 促黄体生成素高峰刺激雄激素的产生，并暴露在促性腺激素高峰。 刺激芳香酶活性。

The overall goal of this project is to determine the causes of follicular atresia in the cycling rat. Anti-testosterone antiserum will be administered to test the hypothesis that accumulation of androgen within the follicle is the immediate cause of atresia. To determine if the LH surge promotes atresia, and prior exposure to FSH prevents atresia, the components of the periovulatory surge of gonadotropins will be dissociated. Porcine follicular fluid ("inhibin") will be used to attenuate the FSH surge; exogenous FSH and LH will be administered to "advance" or augment the endogenous gonadotropin surges. The effect of these treatments on the number and viability of small antral follicles (those follicles particularly prone to atresia), will be examined by histological methods. A time course will be established for the sequence of morphological events associated with atresia in these follicles. Small antral follicles will be isolated and placed in tissue culture to determine if exposure to the LH surge stimulates androgen production, and exposure to the FSH surge stimulates aromatase enzyme activity.