ROLE OF CELL SURFACE ANTIGENS IN MAMMALIAN DEVELOPMENT

拟议研究的基本目标是表征特定阶段的表面。 蛋白质(SSSP)，并研究它们在早期发育和 小鼠胚胎的分化。用小鼠胚胎进行同基因免疫 不同的发育阶段将被用来获得特异性的抗血清。 植入前小鼠胚胎的表面蛋白将被标记， 免疫沉淀与特定的抗血清，并在一和 二维凝胶。来自免疫小鼠的脾细胞将与 产生抗期特异性单抗的骨髓瘤细胞和杂交瘤细胞 分离出表面蛋白。畸胎癌干细胞株将进行测试 检测到抗胚胎血清和具有交叉反应抗原的血清。vbl.使用 单抗和畸胎瘤干细胞，足够量 SSSP将被分离出来并进行详细的表征。抗胚胎血清将是 用来探测胚胎细胞膜的性质并评估 SSSP在胚胎分化发育和畸胎癌中的作用 干细胞在体外。

The basic aim of the proposed research is to characterize stage-specific surface proteins (SSSP) and to examine their role during early development and differentiation of mouse embryo. Syngeneic immunization with embryos of different developmental stages will be used to obtain specific antisera. Surface proteins of preimplantation mouse embryos will be labeled, immunoprecipitated with specific antisera and characterized on one- and two-dimensional gels. Spleen cells from immunized mice will be fused with myeloma cells and hybrids producing monoclonal antibodies against stage-specific surface proteins isolated. Teratocarcinoma stem cell lines will be tested with anti-embryo sera and those with cross-reacting antigens detected. Using monoclonal antibodies and teratocarcinoma stem cells, a sufficient amount of SSSP will be isolated and characterized in detail. Anti-embryo sera will be used to probe the properties of the embryonic cell membrane and to assess the role of SSSP in differentiation and development of embryos and teratocarcinoma stem cells in vitro.