GONADOTROPIN RECEPTOR REGULATION IN VIVO AND IN VITRO

该项目的长期目标是提供一个基本的理解 卵巢细胞促性腺激素受体的调节，最终将 适用于生育调控问题。此应用程序是 关注延续长期项目的三个一般问题 目标并总结/包含本提案的具体目标：a) 某些生长因子在卵巢细胞关键分化中的作用 FSH依赖的促黄体生成素受体诱导等过程，b)是否 促性腺激素和/或性激素可能反过来调节受体 用于生长因子的种群，以及c)使用无血清培养 研究完整细胞中受体调节的条件。具体目标 该项目由以下实验目标组成：1) 血小板衍化生长因子(PDGF)的同种移植试验 卵泡刺激素刺激的促黄体生成素受体诱导及随后的促黄体生成素 猪和大鼠颗粒细胞的受体依赖性细胞功能 单层，2)检查非PDGF成分的可能性 血小板提取物可能促进FSH刺激的促黄体生成素受体的诱导 和/或选择后续的依赖于促黄体生成素受体的细胞功能，3)至 颗粒细胞PDGF受体结合特性及其受体的研究 生殖激素对颗粒细胞单层的调节，4)至 颗粒细胞表皮生长因子受体结合特性的研究 并在体内和体内研究生殖激素对EGF受体的调节 以及5)扩大我们对无血清培养环境和 培养的颗粒细胞的性能通过a)调查 低密度脂蛋白(LDL)和高密度脂蛋白(HDL) 生长因子/促性腺激素刺激的类固醇合成，以及b) 颗粒细胞亚群培养反应的特征 优先富含或缺乏特定的膜受体。

The long-term objective of the project is to provide a basic understanding of ovarian cellular gonadotropin receptor regulation which will eventually be applicable to the problem of fertility regulation. This application is concerned with three general issues which continue the long-term project objective and summarize/encompass the specific aims of this proposal: a) the role of certain growth factors in key ovarian cellular differentiation processes such as FSH-dependent LH receptor induction, b) whether gonadotropins and/or sex steroids might, in turn, modulate receptor populations for growth factors, and c) the use of serum-free culture conditions to study receptor regulation in intact cells. The specific aims of the project consist of the following experimental objectives: 1) to test homogenous prepatations of platelete-derived growth factor (PDGF) upon FSH-stimulated LH receptor induction and upon subsequent LH receptor-dependent cell functions in porcine and rat granulosa cell monolayers, 2) to examine the possibility that a non-PDGF component of platelet extracts might facilitate FSH-stimulated LH receptor induction and/or selected subsequent LH receptor dependent cell functions, 3) to characterize granulosa cell PDGF receptor binding and study PDGF receptor modulation by reproductive hormones in granulosa cell monolayers, 4) to characterize granulosa cell epidermal growth factor (EGF) receptor binding and study EGF receptor modulation by reproductive hormones in vivo and in vitro, and 5) to extend our study of the serum-free culture milieu and the performance of cultured granulosa cells by a) investigating the effects of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) upon growth factor/gonadotropin-stimulated steroidogenesis, and b) characterizing the responses in culture of granulosa cell sub-populations preferentially enriched or lacking in a particular membrane receptor.