MAGNETIC CHARACTERIZATION OF THE WATER OXIDIZING ENZYME

这项工作的目的是了解水是如何被氧化的 在光合作用的有机体中产生氧气。 所有人的健康 包括人类在内的需氧生物， 这是因为它是全球唯一的重要来源。 生物圈中的氧气。 我们试图找出 在此过程中形成的化学中间体结构 四步酶催化反应。 的结构和 四种锰离子的作用， 网站是这项研究的主要目标。 我们引入两 以前没有探索过的研究水的物理方法 光合作用膜中的氧化位点。 有三 建议的研究课题：1）时间分辨磁 化学中间体的敏感性， 光合作用膜中水氧化位点的周转， 2)EPR和ENDOR谱在 确定结合锰的蛋白质氨基酸， 酶簇的活性位点，和3）取代 锰被其他金属离子作为结构和机制 光合作用中水氧化反应的探讨 膜。

The objective of this work is to understand how water is oxidized to from oxygen in photosynthetic organisms. The health of all aerobic organisms, including man, is critically dependent upon this process because it is the only significant global source of atmospheric oxygen in the biosphere. We seek to identify the structures of the chemical intermediates that form during this four step, enzyme catalyzed reaction. The structures of and the roles played by four manganese ions which function at the active site are the key objectives of this research. We introduce two previously unexplored physical methods for studying the water oxidizing site in photosynthetic membranes. There are three proposed topics of research: 1) time resolved magnetic susceptibility of the chemical intermediates which form during turnover of the water oxidizing site in photosynthetic membranes, 2) the application of EPR and ENDOR spectroscopies for determining the protein amino acids which bind manganese in the active site of the enzymatic cluster, and 3) the substitution of manganese by other metal ions as a structural and mechanistic probe of the water oxidizing reaction in photosynthetic membranes.