EPIDERMAL GROWTH FACTOR STIMULATES WOUND HEALING IN VIVO

没有已知的药物可以加速和改善 体内人体伤口愈合的质量。 在初步工作中 重组EGF加快了实验和 人类伤口愈合 EGF的功效是什么？ 可以确定EGF的作用机制， 相关生长因子影响体内伤口愈合。 这 申请将研究介导增长的过程， 哺乳动物皮肤的增殖，因为它们与伤口愈合有关 在体内猪模型中以及在可能的情况下在人类患者中。 其具体目的是：1）扩大组织化学、电镜、 使用EGF及其受体的形态计量学研究 该因子参与猪和人伤口愈合; 2） 以确定细胞的顺序外观和身份 在伤口愈合过程中由EGF和其他生长因子激活 使用结合、磷酸化和原位放射自显影; 3） 通过原位杂交确定EGF-R在伤口愈合过程中 受受体占有率、配体（EGF、TGF-A）或 与其他因子（TGF-β）的相互作用; 4）研究 表皮生长因子及其相关因子对上皮细胞和间质细胞的影响 通过研究连续出现、分布 和细胞外基质成分即胶原蛋白的性质 和纤连蛋白。

No pharmacologic agent is known to accelerate and improve the quality of human wound healing in vivo. In preliminary work recombinant EGF accelerated the quality of experimental and human wound healing. Now that milligram quantities of EGF are available it is possible to determine mechanisms whereby EGF and related growth factors affect wound healing in vivo. This application will study processes mediating growth and proliferation of mammalian skin as they relate to wound healing in an in vivo porcine model and where possible in human patients. The specific aims are: 1) To extend histochemical, EM and morphometric studies using EGF and its receptor to define involvement of this factor in porcine and human wound healing; 2) To determine the sequential appearance and identity of cells activated during wound healing by EGF and other growth factors using binding, phosphorylation, and in situ autoradiography; 3) To determine by in situ hybridization if EGF-R during wound healing is modulated by receptor occupancy, ligand (EGF, TGF-A) or interactions with other factors (TGF-B); 4) To study the effects of EGF and related factors on epithelial and mesenchymal interactions by studying the sequential appearance, distribution and nature of extracellular matrix components namely collagens and fibronectin.