GONADOTROPIN ACTION IN FOLLICLES AND CORPORA LUTEA

我们的长期研究目标是进一步了解cAMP 卵巢卵泡和黄体细胞的反应机制： 促黄体生成激素（LH）、卵泡刺激素（FSH） 刺激激素（FSH）和类固醇激素，如雌激素; 在卵泡和黄体中的生理意义。 在 腺苷酸环化酶的水平，我们建议评估 Mg ~（2+）和LH诱导细胞脱敏的分子机制 腺苷酸环化酶转化为LH的无细胞膜制剂， 排卵前猪卵泡。 在cAMP依赖性蛋白水平上， 激酶，我们建议确定的生化特性的 调节和催化蛋白激酶亚基; 调节和催化亚基活性以及调节亚基水平 LH、FSH和雌激素;以及蛋白激酶的激素调节 亚基结合形成全酶。 这些蛋白激酶 将在卵泡和黄体中跟踪参数， 在大鼠和家兔生殖阶段的特定时间。 方法 包括腺苷酸环化酶、磷酸转移酶cAMP 结合活性; Protesterone和cAMP放射免疫测定;标准 蛋白质纯化技术和重组DNA技术。 是 通过对这些关键酶的分子调控的研究， 我们可以理解的机制， 促卵泡激素和雌激素在卵巢中的作用 卵泡和黄体。

Our long-term research goal is to further our understanding of the cAMP response mechanisms in ovarian follicular and luteal cells: their regulation at the molecular level by luteinizing hormone (LH), follicle stimulating hormone (FSH) and steroid hormones like estrogen; their physiological significance in the ovarian follicle and corpus luteum. At the level of the adenylyl cyclase enzyme, we propose to evaluate the molecular mechanism by which both Mg2+ and LH induce desensitization of the adenylyl cyclase to LH in a cell-free membrane preparation derived from preovulatory porcine follicles. At the level of the cAMP-dependent protein kinase enzyme, we propose to determine the biochemical properties of the regulatory and catalytic protein kinase subunits; the regulation of the regulatory and catalytic subunit activities and regulatory subunit levels by LH, FSH and estrogen; and the hormonal regulation of protein kinase subunit associations to form the holoenzymes. These protein kinases parameters will be followed in ovarian follicles and corpora lutea at specified times during the reproductive phases of rat and rabbits. Methods include standard assays for adenylyl cyclase, phosphotransferase cAMP binding activities; protesterone and cAMP radioimmunoassays; standard protein purification techniques; and recombinant DNA technology. It is through such studies on the molecular regulation of these key enzyme systems by the gonadohormones that we can understand the mechanism of action and therefore the functions of LH, FSH and estrogen in ovarian follicles and corpora lutea.