SPATIAL PATTERN OF THE EGG CHAMBER IN DROSOPHILA

这个项目的目的是分析空间不对称性如何 出现在果蝇发育中的卵室中。四个基因 (尖顶、卡布奇诺、格尔肯和鱼雷) 蛋壳和蛋壳的正确空间图案 将对胚胎进行分析。其中三个基因是细胞所必需的 而鱼雷在体细胞中起作用。这个 因此，分析集中在卵子发生的一个阶段，当两者都 蛋壳的图案和胚胎的图案 取决于不同卵巢细胞之间的相互作用 类型。 为了更准确地定义这四个基因在生殖系中的作用 体细胞、新等位基因和染色体重排 将被分离，并对野生型和 突变卵室将在整个架子上进行研究 剖切材质。对马赛克卵室的分析 鱼雷以及显性和隐性标记突变将 用来研究卵泡细胞在卵巢癌中的作用 卵室的图案。一种初步的分子分析方法 尖顶和鱼雷将被执行。这将包括一个 基因组走到包含这两个基因座的染色体区域， 对基因进行鉴定，并对基因的空间和空间分布进行分析 他们的产品的时间分布。这将使我们能够 发展一个作用于花纹形成机制的分子模型 卵室。

The aim of this project is the analysis of how spatial asymmetries arise in the developing egg chamber of Drosophila. Four genes (spire, cappuccino, gurken, and torpedo) which are required for the correct spatial patterning of both the egg shell and the embryo will be analyzed. Three of the genes are required in cells of the germline, wheras torpedo functions in somatic cells. The analysis focusses therefore on a stage in oogenesis when both the pattern of the egg shell and the pattern of the embryo are dependent on interactions between the different ovarian cell types. To define more precisely the role of the four genes in germline and somatic cells, new alleles and chromosomal rearrangements at the loci will be isolated, and the morphology of wildtype and mutant egg chambers will be studied in whole mounts and sectioned material. Analysis of mosaic egg chambers using torpedo, as well as dominant and recessive marker mutations, will be used to investigate the role of the follicle cells in the patterning of the egg chamber. An initial molecular analysis of spire and torpedo will be performed. This will consist of a genomic walk to the chromosomal region containing the two loci, identification of the genes, and an analysis of the spatial and temporal distribution of their products. This will allow us to develop a molecular model of patterning mechanisms acting in the egg chamber.