PICOSECOND CIRCULAR DICHROISM STUDIES OF PROTEIN MOTION

时间分辨圆二色谱是我们拥有的一种技术 我们实验室为研究蛋白质构象而开发的 动力学。我们试验性方法的能力使人们能够 测量发生在皮秒或微秒内的蛋白质的构象运动 较慢的时间尺度。 在这个提案中，我们详细讨论了实验方法 皮秒时间上时间分辨圆二色谱的测量 我们的技术在蛋白质研究中的规模和应用 底物光诱导脱脂引起的构象变化 结合肌红蛋白、血红蛋白和模型系统。此外，我们还讨论了 我们新的实验技术在未来的应用(1) 视网膜异构化在视觉上引起的蛋白质运动 蛋白质视紫红质，以及(2)电子引起的蛋白质结构变化 杂交血红蛋白中的转移化学。

Time resolved circular dichroism spectroscopy is a technique that we have developed in our laboratory for the study of protein conformational dynamics. The capabilities of our experimental approach allows one to measure conformational motions of proteins that occur on the picosecond or slower time scale. In this proposal, we discuss, in detail, the experimental approach for measuring time resolved circular dichroism spectra on the picosecond time scale and applications of our technique to the study of protein conformation changes caused by the photoinduced debinding of substrates bound to myoglobin, hemoglobin and model systems. In addition, we discuss future applications of our new experimental technique to determine (1) protein motions induced by the isomerization of retinal in the visual protein rhodopsin, and (2) changes in protein structure caused by electron transfer chemistry in hybrid hemoglobins.