ORGANIC SECONDARY ION IMAGING

这项研究的目的是开发一种确定 靶向有机化合物在生物组织中的空间分布。 这种能力将使快速分析组织成为可能 毒素和药物的本地化，很可能是它们的 代谢物。二次离子质谱仪(SIMS)用于 获取组织的有机离子显微照片。空间分辨率 能否达到将取决于二次元的实际产量 离子，即检测到的分析物特征二次离子的数量 每个入射的初级离子。仪器功能将通过以下方式进行优化 将离子光学设计标准严格应用于整个次级 离子质谱仪。化学原位预处理法的研究 将开发样品以增强特征二次离子 选定分析物的排放。初级离子束物种的选择和 将研究优化二次离子产率的条件。串列 将采用质谱学(MS/MS)，以便特征离子可以 除了它们的质荷比外，还可以通过它们的结构来分解， 就像目前在模拟人生中所做的那样。数据将使用现有的 IBM PC/XT数据系统，具有离线数据处理和图像处理功能 由DEC MicroVAX计算机执行。

The objective of this research is development of a method to determine spatial distributions of targeted organic compounds in biological tissue. Such a capability would make possible rapid analysis of tissue for localization of toxins and pharmaceuticals, and quite possibly, their metabolites. Secondary ion mass spectrometry (SIMS) will be employed to obtain organic ion micrographs of the tissue. The spatial resolution which can be attained will be determined by the practical yield of secondary ions, that is, the number of analyte-characteristic secondary ions detected per incident primary ion. Instrumental capabilities will be optimized by rigorous application of ion optical design criteria to the entire secondary ion mass spectrometer. Methods of in situ chemical pretreatment of the sample will be developed for enhancing characteristic secondary ion emission from selected analytes. Choice of primary ion beam species and conditions will be investigated to optimize secondary ion yield. Tandem mass spectroscopy (MS/MS) will be employed so that characteristic ions can be resolved by their structure, in addition to their mass-to-charge ratios, as is currently practiced in SIMS. Data will be obtained using an existing IBM PC/XT data system, with off-line data manipulation and image processing performed by a DEC MicroVAX computer.