TESTIS HORMONES AND GONADOTROPINS IN MALE INFERTILITY

描述(改编自申请人的摘要)：长期目标 本申请中建议的研究是检查相互作用 睾丸激素与脉动性促性腺激素分泌的关系 努力更好地了解人类男性生育和生殖的机制 不孕不育。研究人员计划检查搏动性黄体生成。 正常男性激素和糖蛋白α亚单位分泌， 睾丸切除男性、睾丸衰竭男性和特发性男性 使用强化采血方案进一步了解不孕症 促性腺激素释放激素分泌的生理学。有人建议进行研究，以确定 脉冲促性腺激素分泌通过重复采样对睾丸的影响 精索静脉血中睾酮及其代谢产物的测定 精索静脉曲张相关性不育症患者的前体类固醇。他们的 研究计划还包括使用分散的大鼠脑垂体细胞刺激 以促性腺激素释放激素的脉冲为模型，模拟了 下丘脑-垂体单位探索与人类相关的问题 不能直接在人类身上研究的病理生理学。这些研究可能 阐明睾丸激素如何直接影响促性腺激素的释放 不依赖于它们对促性腺激素释放激素的作用。具体来说， 他们打算研究雄激素和抑制素对FSH的反馈作用， 正常和去势垂体腺细胞分泌黄体生成素和α-亚基 雄鼠。抑制素将从灵长类支持细胞培养中获得 5~6成熟。因为人类男性不育是一种常见的疾病，没有 统一有效的治疗，这些研究可能会提供重要的 向生理学领域的临床医生和研究人员提供信息， 内分泌学、生物化学、泌尿学和妇科。

DESCRIPTION (Adapted from the applicant's abstract): The long-term goal of the proposed studies in this application is to examine the interactions between testicular hormones and pulsatile gonadotropin secretion in an effort to better understand the mechanisms of human male fertility and infertility. The investigators plan to examine pulsatile luteinizing hormone and glycoprotein alpha-subunit secretion in normal men, orchidectomized men, men with testicular failure, and men with idiopathic infertility using intensive blood sampling protocols to understand further the physiology of GnRH secretion. Studies are proposed to determine how pulsatile gonadotropin secretion effects the testis by repetitive sampling of spermatic vein blood for the measurement of testosterone and its precursor steroids in men with varicocele-associated infertility. Their study plan also includes use of dispersed rat pituitary cells stimulated with pulses of GnRH as a model which simulates the normal function of the hypothalamic-pituitary unit to explore questions relevant to human pathophysiology which cannot directly be studied in man. These studies may clarify how testis hormones influence directly the release of gonadotropins by pituitary cells independent of their actions on GnRH. Specifically, they intend to study the feedback actions of androgens and inhibin on FSH, LH and alpha-subunit release by pituitary cells from normal and castrated male rats. Inhibin will be obtained from primate Sertoli cell culture medium. Because human male infertility is a common disorder with no uniformly effective treatment, these studies may provide important information to clinicians and investigators in the fields of physiology, endocrinology, biochemistry, urology and gynecology.