PLASMA MEMBRANE CHANGES IN FERTILIZATION

这项研究的总体目标是描述 海胆卵的细胞表面，并阐明它们的作用 受精过程中初始事件中的成分。1)我们将继续 从鸡蛋中分离的一种细胞表面复合体的成分研究 由质膜组成，外层涂覆有外周 卵黄层和内侧皮质层的蛋白质 水泡。在对卵黄成分进行进一步细分后， 质膜、皮质囊泡膜和 将分离皮质小泡，并对其进行对比分析 多肽、脂类和标记酶。对…有更清楚的了解 受精后这些成分的命运将被比较 透明层和受精膜的已知成分。 2)由质膜制备的囊泡的离子渗透性， 皮质囊泡膜和由两者组成的马赛克膜 将被测量以确定是否这些膜中的一个负责 观察到Ca~(++)和Na~+向卵子内流的增强 受精。3)我们将研究钙离子依赖的可能性 分解和花生四烯酸释放。细胞表面复合体可以是 研究动力学和函数关系的极佳模型 分泌和花生四烯酸的产生和代谢。4)结构 将对蛋冻涂层中的硫酸多糖进行研究，以确定 导致物种特异性诱导的结构差异 精子的顶体反应。用果冻皮作亲和配基 将负责分离精子表面的蛋白质 它的功能是作为果冻涂层的受体。的属性和模式 分离的受体在诱导顶体反应中的作用将是 学习。5)使用结合的，或针对糖肽制备的抗体 以受体片段为工具，我们将分离精子的受体 从溶解的卵细胞表面膜中提取。纯化的受体将是 它的特征和结构组件对这两个 将定义精子粘附性和物种专一性。

The overall objective of this study is to characterize the components of the cell surface of the sea urchin egg and to elucidate the role of these components in the initial events in fertilization. 1) We will continue studies on the components of a cell surface complex isolated from eggs that consists of the plasma membrane, coated on the outer side with peripheral proteins of the vitelline layer and on the inner side with cortical vesicles. Following further subfractionation the vitelline components, plasma membrane, cortical vesicle membrane, and the contents of the cortical vesicles will be isolated, and analyzed comparatively for polypeptides, lipids, and marker enzymes. To obtain a clearer picture of the fate of these components following fertilization, they will be compared with known components of the hyaline layer and the fertilization envelope. 2) The ion permeability of vesicles prepared from the plasma membrane, the cortical vesicle membrane and a mosaic membrane consisting of both of them will be measured to determine if one of these membranes is responsible for the enhanced influx of Ca++ and Na+ into the egg observed upon fertilization. 3) We will investigate the possibility that Ca++ dependent breakdown and arachidonic acid release. The cell surface complex may be an excellent model to study the kinetic and functional relationships between secretion and arachidonic acid production and metabolism. 4) The structure of the sulfated polysaccharides in egg jelly coat will be studied to define the structural differences that result in species specific induction of the acrosomal reaction in sperm. Using jelly coat as an affinity ligand we will undertake the isolation of the protein on the surface of the sperm that functions as a receptor of jelly coat. The properties and mode of action of the isolated receptor in inducing the acrosomal reaction will be studied. 5) Using binding, or antibody prepared towards a glycopeptide fragment of the receptor as tools, we will isolate the receptor for sperm from solubilized egg cell surface membranes. The purified receptor will be characterized and the structural components of it responsible for both sperm adhesion and species specificity will be defined.