FOLLICLE SELECTION: A CELL KINETIC STUDY

早期窦期是卵泡发育的关键点， 循环率 卵泡只有两条路离开这个阶段：一些继续前进， 成熟为排卵做准备;所有其他退化（经历 闭锁）。 虽然FSH激增在决定哪些因素中起着重要作用， 卵泡将排卵，其确切的肌动蛋白机制尚不清楚。 这里提出的研究将测试假设， FSH的浓度是需要在早期窦阶段， 发育以启动颗粒细胞的分化。 因为 分化过程与细胞分裂的结束有关， 细胞增殖的动力学应该随着选择过程而改变， 会发生 我将：（1）充分表征颗粒的动力学 使用细胞周期分析技术进行细胞生长， 卵泡发育的决定点。 (2)测量多个参数 颗粒细胞增殖（生长分数，出生率，世代 时间，同步性），并测定血清FSH浓度， FSH分泌的变化与颗粒细胞生长速率的变化。 (3)实验性改变内源性FSH浓度（用猪 卵泡液或半去势）来直接检验假设， FSH调节颗粒细胞增殖。 这些研究将开始， 明确选择一个卵泡排卵的精确机制 而另一个则被流放到阿斯特雷西亚。

The early antral stage is a pivotal point in follicular development in the cyclic rat. Follicles leave this stage by only two paths: some go on to mature in preparation for ovulation; all others degenerate (undergo atresia). Although the FSH surge plays a major role in determining which follicles will ovulate, its precise mechanism of actin remains unclear. The research proposed here will test the hypothesis that high concentrations of FSH are required during the early antral stage of development to initiate differentiation of the granulosa cells. Because the process of differentiation is associated with the end of cell division, the dynamics of cell proliferation should change as the selection process takes place. I will: (1) fully characterize the dynamics of granulosa cell growth using techniques of cell cycle analysis to identify this decision point in follicular development. (2) measure several parameters of granulosa cell proliferation (growth fraction, birth rate, generation time, synchrony) and determine serum FSH concentrations to correlate cyclic variation in FSH secretion with changes in granulosa cell growth rates. (3) experimentally alter endogenous FSH concentrations (with porcine follicular fluid or hemicastration) to directly test the hypothesis that FSH regulates granulosa cell proliferation. These studies will begin to define the precise mechanism by which one follicle is chosen for ovulation while another is relegated to astresia.