NEUTRAL PROTEINASES IN ENDOCHONDRAL BONE FORMATION

该项目旨在探索神经性蛋白水解酶在 生长区伴随骨形成的软骨基质的去除 胚胎肢体原基。建议的工作假设 研究表明，神经蛋白多糖降解酶对组织具有抵抗力 和血清抑制物，类似于一种由恶性老鼠肿瘤分泌的 我们最近描述的酶可能参与了正常的过程。 胚胎软骨摘除伴随软骨内骨形成。 对鸡胚胎肢体的初步研究表明，存在 生长区中的蛋白多糖降解酶，其中之一可能 具有与肿瘤酶相似的性质。 1.以肿瘤酶为抗原的免疫定位方法 将在单抗中搜索类似的酶 大鼠胚胎长骨和骨修复组织的生长区 新生大鼠。 2.发育鸡胚肢，可提供更多的组织 生化研究，将用于分离、纯化和 中性PG降解酶的特性研究。 3.这些酶的细胞来源、调控及其与骨的关系 将使用生化和免疫学来探索形成和修复 方法建立在本实验室的基础上。 所获得的知识可能不仅有助于理解正常 骨骼发育，但也包括遗传和代谢异常以及骨骼 维修流程。

This project is designed to explore the role of neural proteinases in the removal of cartilage matrix accompanying bone formation in the growth zones of embryonic limb primordia. The working hypothesis for the proposed studies is that neural proteoglycan degrading enzymes resistant to tissue and serum inhibitors, similar to one secreted by a malignant rat tumor enzyme recently described by us may be involved in the normal process of cartilage removal accompanying endochondral bone formation in the embryo. Preliminary studies in the chick embryo limb indicate the presence of proteoglycan degrading proteinases in the growth zone, one of which may have properties similar to that of the tumor enzyme. 1. By immunolocalization methods using the tumor enzyme as antigen for monoclonal antibodies a search will be made for similar enzymes in the growth zones of embryonic rat long bones and in bone repair tissue in neonatal rats. 2. Developing chick embryo limb, which can provide more tissue for biochemical studies, will be used for isolation, purification and characterization of neutral PG degrading proteinases. 3. Cellular source, regulation and relationship of these enzymes to bone formation and repair will be explored using biochemical and immunological methods well established in this Laboratory. The knowledge gained may be useful in understanding not only normal skeletal development, but also genetic and metabolic abnormalities and bone repair processes.