HCG IN NORMAL HUMANS

我们已经开发出非常敏感和特异的双克隆， 人促甲状腺激素抗体夹心式测定法 促黄体生成素、人绒毛膜促性腺激素 人催乳素。使用我们已有的hCG和HLH的分析方法 所有正常未孕妇女未提取血中hCG的定量 女性、绝经后女性和男性。我们的数据表明hCG 由脑下垂体分泌。HCG被分泌在 在女性中，与荷尔蒙激素平行的脉动时尚。我们现在提议 继续在两个大的领域进行研究：(A)继续进行技术研究 人促卵泡激素ACTH的检测进展 (HFSH)和人α链，以及(B) 正常人体内分泌的“类hCG”物质。这包括 如下：(1)脉动动力学和 人绒毛膜促性腺激素在正常人和精神障碍患者中的控制 下丘脑-垂体-性腺系统；(2)测定 正常人脑垂体型hCG分泌；(3)物理化学 猪瘟病毒氨基酸序列的鉴定与测定 从正常人脑垂体、血浆和尿液中提纯人绒毛膜促性腺激素； 氨基酸的理化性质及测定方法 细菌产生的类hCG物质的序列 与正常人类产生的病毒相比。

We have developed very sensitive and specific, two-monoclonal, antibody sandwich type assays for human thyrotropin, human luteinizing hormone (hLH), human chorionic gonadotropin (hCG), and human prolactin. Using the assays for hCG and hLH we have quantified hCG in unextracted blood of all normal non pregnant women, post-menopausal women, and men. Our data suggest hCG is secreted by the pituitary gland. and that hCG is secreted in pulsatile fashion in parallel with hLH in women. We now propose continued research in two broad areas: (A) continued technical assay development - for ACTH, human follicle stimulating hormone (hFSH), and human alpha chain, and (B) characterization of the "hCG-like" material secreted in normals. This includes the following: (1) characterization of the pulsatile dynamics and control of hCG in normal humans and humans with disorders of the hypothalamic-pituitary-gonadal system; (2) determination of the pituitary cell-type secreting hCG in normals; (3) physico-chemical characterization and determination of the amino acid sequence of hCG purified from pituitary, plasma and urine of normals; (4) physico-chemical characterization and determination of amino acid sequence of the "hCG-like material produced by bacteria with comparison to that produced by normal humans.