REPRODUCTION: PHOTIC-NEUROENDOCRINE CONTROL OF PROLACTIN

我们的目标是阐明和理解的基本机制 潜在的长期光神经内分泌控制催乳素（PRL） 季节性繁殖的雄性叙利亚仓鼠（Mesocricetus）的细胞功能 auratus）。 这项研究也旨在更好地了解 松果体的作用，在调解的 光照-神经内分泌控制PRL细胞活性。 初步研究 将确定短光周期对PRL合成，储存， 在性腺退化的早期阶段降解和分泌。 PRL合成的变化将通过测量 3 H-亮氨酸转化为新合成的催乳素，以及通过 PRL信使RNA的体内水平的测量。 PRL存储将是 通过测量免疫反应性RIA-PRL的总量， 含有来自每个处理组的垂体的孵育小瓶。 PRL 体内释放将通过测量免疫反应性的量来评估。 各给药组仓鼠血清中的RIA-PRL。 的 将通过脉冲追踪标记技术评估PRL的降解。 其他研究将确定短光周期对 从仓鼠茎中值释放PRL调节因子 隆起（SME）。 这些影响将与 短光周期对PRL细胞活性的影响，特别是在 性腺退化的阶段 实验也将致力于 确定PRL细胞对一种或多种细胞因子的敏感性是否改变， PRL-监管暴露。 将进行平行研究， PRL细胞对已知的下丘脑PRL调节因子的敏感性改变 因子（多巴胺、γ-氨基丁酸、促甲状腺激素释放因子 激素）将响应于短光周期来测量。 在所有 建议研究松果体的作用，在调解短 光周期诱导的PRL细胞功能变化将通过以下方法确定： 包括切除松果体的仓鼠。 对长期的理解 在这个物种中对PRL的抑制控制可能会导致新的 长期抑制异常高PRL水平的方法， 患有下丘脑-垂体疾病和生殖功能改变的患者 功能

The goal is to elucidate and understand the fundamental mechanisms underlying the long term photic-neuroendocrine control of prolactin (PRL) cell function in the seasonally-breeding male Syrian hamster (Mesocricetus auratus). The research is also aimed at achieving a better understanding of the pineal gland's role in the mediation of the photoperiodic-neuroendocrine control of PRL cell activity. Initial studies will determine the effects of short photoperiod on PRL synthesis, storage, degradation and secretion during the early stages of gonadal regression. Changes in PRL synthesis will be assessed by measuring the incorporation of 3H-leucine into newly synthesized PRL in vitro as well as by the measurement of in vivo levels of PRL messenger RNA. PRL storage will be determined by measuring the total amount of immunoreactive RIA-PRL in the incubation vials containing pituitaries from each treatment group. PRL release in vivo will be assessed by measuring the amount of immunoreactive RIA-PRL in the serum of hamsters from each treatment group. The degradation of PRL will be assessed via pulse-chase labelling techniques. Other studies will determine the effects of short photoperiod on the release of a PRL-regulatory factor(s) from the hamster stalk median eminence (SME) in vitro. These effects will be correlated with the effects of short photoperiod on PRL cell activity, particularly during the early stages of gonadal regression. Experiments will also be devoted to the determination of whether alterations in PRL cell sensitivity to a PRL-regulatory exposure. Parallel studies will be conducted in which changed in PRL cell sensitivity to known hypothalamic PRL-regulatory factors (dopamine, Gamma amino butyric acid, thyrotrophin-releasing hormone) will be measured in response to short photoperiod. In all of the proposed studies the role of the pineal gland in mediating short photoperiod-induced changes in PRL cell function will be determined by including pinealectomized hamsters. An understanding of the long term inhibitory control of PRL in this species could potentially lead to new approaches to the long term suppression of abnormally high PRL levels in patients with hypothalamic-pituitary disorders and altered reproductive function.