CHOLESTEROL INTERACTIONS: SERUM AND MEMBRANE PROTEINS

这个项目探讨了胆固醇相互作用的基本未开发的概念 蛋白质。 一些间接的测量表明，这种相互作用可能 在人低密度脂蛋白（LDL）和带3的结构中是重要的 蛋白质（阴离子转运蛋白）从人类红细胞膜。 因为 LDL和胆固醇在动脉粥样硬化发病机制中的作用， 重要的是要探索胆固醇在其两个位置的行为， 人血、低密度脂蛋白和红细胞膜。 发现 与蛋白质相互作用的可能性将显著改变 胆固醇在膜和脂蛋白中的行为。 这一提议通过直接的、非扰动的方法（首次）进行探测， 新的间接和有点扰动的方法， 胆固醇与蛋白质：1）LDL，2）红细胞膜。 两 将检查红细胞膜蛋白; MN-糖蛋白 （血型糖蛋白）和带3蛋白。 31 P NMR、2D NMR、ESR和CD测量将 被探索。 这些结果将被用来更全面地描述LDL的结构和LDL的代谢。 红细胞膜，以及这些系统中的脂质-蛋白质相互作用。 的 信息应证明有价值的了解运输的 胆固醇和动脉粥样硬化斑块的发展。

This project explores the largely unexplored concept of cholesterol interaction with proteins. Some indirect measures have suggested that such interactions may be important in the structure of human low density lipoprotein (LDL) and band 3 protein (anion transport protein) from the human erythrocyte membrane. Because of the roles of LDL and cholesterol in the pathogenesis of atherosclerosis, it is important to explore the behavior of cholesterol in two of its locations in human blood, low density lipoproteins and the erythrocyte membrane. Discovery of an interaction with protein would significantly alter the common view of cholesterol behavior in membranes and lipoproteins. This proposal probes by direct, non-perturbing methods (for the first time) and new indirect and somewhat perturbing methods the possible interaction of cholesterol with protein in: 1) LDL, and 2) the erythrocyte membrane. Two proteins from the erythrocyte membrane will be examined; MN-glycoprotein (glycophorin) and band 3 protein. 31P NMR, 2D NMR, ESR and CD measurements will be explored. The results will be employed to describe more fully the structure of LDL and the erythrocyte membrane, and lipid-protein interactions in these systems. The information should prove valuable to the understanding of the transport of cholesterol and the development of the placques of atherosclerosis.