ROLE OF PLASMA LIPOPROTEINS IN ATHEROSCLEROSIS

拟议研究的最终目标是澄清这些关系 血浆脂蛋白与动脉粥样硬化之间的关系。我们将通过以下方式实现这一目标 考察了各种物理化学和酶促因素对酶活性的影响。 血浆脂蛋白及其脂类在动脉中的滞留和蓄积 组织通过利用模型系统。由于高脂血症具有抗动脉粥样硬化作用 密度脂蛋白(Hdl)可能与卵磷脂-胆固醇有关。 酰基转移酶反应，我们打算提供更多关于基础的信息 人血浆卵磷脂-胆固醇酰基转移酶的性质 理化和免疫化学性质。我们将研究以下方面的作用 酶-脂蛋白相互作用中的各种载脂蛋白。我们还打算 大鼠和猪血清中酶的顺序纯化方法的建立 为了便于研究酶反应和 实验动物的高密度脂蛋白代谢。此外，我们还将继续研究 血浆脂蛋白与主动脉结缔组织相互作用的研究进展 成分，如糖胺多聚糖、蛋白多糖、弹性蛋白和胶原蛋白。 对这些相互作用的影响，发生在地表和 脂蛋白在循环过程中和循环后的内部性质 将对进入大动脉的情况进行系统调查。

The ultimate goal of the proposed research is to clarify the relationships between plasma lipoproteins and atherosclerosis. We will approach this goal by investigating various physicochemical and enzymatic factors influencing the retention and accumulation of plasma lipoproteins and their lipids in arterial tissues by utilizing model systems. Since antiatherogenic effects on high density lipoproteins (HDL) may well be associated with the lecithin-cholesterol acyltransferase reaction, we intend to provide further information on the basic properties of human plasma lecithin-cholesterol acyltransferase, especially its physicochemical and immunochemical properties. We will study the roles of various apo-lipoproteins in the enzyme-lipoprotein interactions. We also intend to establish the method for enzyme purification from rat and swine sera in order to facilitate the study of the relationship between the enzymatic reaction and HDL metabolism in experimental animals. In addition, we will continue the study of the interactions of plasma lipoproteins with aortic connective tissue components, such as glycosaminoglycans, proteoglycans, elastin, and collagen. The effects, on these interactions, of the changes occurring in the surface and interior properties of the lipoproteins during their circulation and after their entry into the aorta will be systematically investigated.