CAPILLARY AND INTERSTITIAL FLUID AND SOLUTE EXCHANGE

血浆蛋白在血管外的分布受以下因素影响 血管屏障的选择性和相互关联的 胶原蛋白和糖胺多聚糖的基质 间质。在我们目前的工作中，我们发现 间质内白蛋白和免疫球蛋白在血管外的质量 水肿与淋巴蛋白浓度的变化不平行。 在皮肤和骨骼肌中都有。我们的假设是血浆 蛋白质分布在两个隔室中：一个非限制性的 与淋巴自由交换的人和有限制的人 它在空间和静电上限制了运动， 血浆蛋白的分布。有一种跨血管蛋白 流入两个隔室；然而，进入 限制性隔室以扩散为主，而 进入淋巴室的主要是对流。这个 进入限制性隔室的血管转运取决于 主要依靠血浆蛋白的电荷，而进入 淋巴室取决于大小。经济衰退带来的影响 对蛋白质运输的限制更多 主要存在于皮肤中，胶原含量较高，而不是骨骼 肌肉。示踪白蛋白和免疫球蛋白的比活度将为 在术后不同时间对淋巴和组织进行测量 恒定的血浆活度以确定相对大小和 两个车厢的周转率。来自皮肤的数据将是 与来自骨骼肌的数据进行比较，以确定这两个 隔室行为在皮肤中更占优势。数据 血浆置换后获得的数据将与数据进行比较 在静脉压升高后获得验证假说 降低血浆肿胀压会影响限制性 当微血管压力增加时，隔室会影响 非限制性隔间。单体的平衡化 以及不同等电点的二聚体白蛋白 确定电荷在血管外的重要性 血浆蛋白的分布。其初始组织摄取率 不同等电点的单体和二聚体蛋白 会被比作稳态的淋巴到血浆 确定浓缩率之间的关系 电荷、微血管选择性和淋巴浓度。 这些结果将提供有关 组织液平衡中的间质基质及其相互关系 淋巴蛋白浓度与微血管的关系 选择性。

The extravascular distribution of plasma proteins is effected by both the selectivity of the vascular barrier and an interlinked matrix of collagen and glycosaminoglycans within the interstitium. In our present work, we found that changes in the extravascular mass of albumin and IgG following interstitial edema did not parallel changes in the lymph protein concentration in both skin and skeletal muscle. Our hypothesis is that plasma proteins distribute within two compartments: a non-restrictive one which exchanges freely with lymph and a restrictive one which sterically and electrostatically restrict the movement and distribution of plasma proteins. There is transvascular protein flux into both compartments; however, the protein flux into the restrictive compartment is predominately diffusion, while that into the lymph compartment is predominately convective. The transvascular transport into the restrictive compartment depends mainly upon the charge of the plasma protein, while that into the lymph compartment depends upon size. The effects of the restrictive compartment on protein transport are more predominate in skin, with its high collagen content, than skeletal muscle. The specific activity of tracer albumin and IgG will be measured in lymph and tissue at different times following a constant plasma activity to determine the relative sizes and turnover rates of the two compartments. Data from skin will be compared to data from skeletal muscle to determine if the two compartment behavior is more predominant in skin. Data obtained following plasmapheresis will be compared to data obtained after increased venous pressure to test the hypothesis that decreasing plasma oncotic pressure effects the restrictive compartment while increasing microvascular pressure effects the non-restrictive compartment. The equilibration of monomeric and dimeric albumin of different isoelectric points will be studied to determine the importance of charge on the extravascular distribution of plasma proteins. The initial tissue uptake rate of monomeric and dimeric albumins of different isoelectric points will be compared to the steady-state lymph-to-plasma concentration ratios to determine the relationship between charge, microvascular selectivity, and lymph concentrations. These results will provide information on the role of the interstitial matrix in tissue fluid balance and the relationship between lymph protein concentrations and microvascular selectivity.