NMR STUDIES IN NORMAL AND DISEASES HUMAN TISSUES

31P核磁共振为非侵入性肌肉生化研究提供了一种独特的方法 正常患者和疾病患者。10 1/4“净孔核磁共振波谱仪 提供了一个机会来评估四肢的肌肉生化 正常的和患病的人类。肌肉生物能量学的主要变化 在方便的运动范围内，磷酸肌酸(PCr)会减少 从大约6倍的ATP水平到1倍的APT水平 无机磷的相应上升几乎保持不变。 聚合酶链式反应/多聚酶链式反应(或聚合酶链式反应/(聚合酶链式反应+多聚酶链式反应))评价生物化学功能 休息和锻炼人的前臂和小腿肌肉，并评估 组织的供氧能力与线粒体的能力 动员底物和利用氧气保持较高的PCR水平 和圆周率低点。31P核磁共振研究中误差性质的研究 人体组织在此应用于静息状态下的人类腿部 正常对照组和单侧糖尿病患者的运动状况 外周血管疾病。数据的可重复性和可靠性 在这些条件下来自这些组将提供使用的基线 作为临床分析生化工具的核磁共振；腿是器官 大多数在各种患病的州都有，这些数据将支付 为将来应用于其他身体器官提供了基准。因此，这些协议 涉及适当的休息状态，最大限度的肢体锻炼对 特定的受试者，无论是正常的还是患病的，然后插入肢体 在一次定量练习后立即输入核磁共振波谱仪 养生法。确定了此过程的恢复时间类别 并确定了它们的可靠性。在这个数据库上，核磁共振的可靠性将 通过适当的统计方法确定，并与 通过其他方法获得的疾病类别。航向的预测 外周血管疾病及其治疗效果的研究 通过核磁共振技术进行的程序最终可能成为可能。

31P NMR presents a unique approach to non-invasive muscle biochemistry in normal and diseased patients. A 10 1/4" clear bore NMR spectrometer affords an opportunity to evaluate muscle biochemistry in the limbs of normal and diseased humans. The principal changes of muscle bioenergetics over the convenient range of exercise are phosphocreatine (PCr) decrease from approximately 6 times the ATP level to one times the APT level with corresponding rises of inorganic phosphate remaining practically constant. PCr/Pi (or PCr/(PCr + Pi)) assesses the biochemical function of the resting and exercised human forearm and calf muscle and evaluates the competency of oxygen delivery to tissue and the capability of mitochondria to mobilize substrates and utilize oxygen for maintaining PCr levels high and the Pi low. A study of the nature of errors in the 31P NMR study of human tissues is here applied to the human leg under resting and exercise conditions in normal controls and in patients having unilateral peripheral vascular disease. The repeatability and reliability of the data from these groups under these conditions will afford a baseline for the use of NMR as a clinical analytical biochemical tool; the leg being the organ most available in a variety of diseased states, and the data will afford a baseline for future applications to other body organs. Thus the protocols involve appropriate resting states, maximum limb exercise feasible for the particular subject, normal or diseased, followed by insertion of the limb into the NMR spectrometer immediately following a quantitative exercise regimen. Categories of recovery times from this procedure are established and their reliability determined. On this data base, NMR reliability will be established by appropriate statistical methods, with correlations with disease categories obtained from other methods. Prediction of the course of peripheral vascular disease and the effectiveness of therapeutic procedures by the NMR technique may ultimately be possible.