LIPID INVOLVEMENT IN OXYGEN TOXICITY

有毒的氧物种与导致肺损伤有关。有证据表明 氧自由基和其他氧物种的参与是推断的，但 基于目前对复杂生物中氧化学的理解 系统。重要的是要了解有毒氧物种如何导致 组织损伤以及它们通过什么化学机制起作用。 总的来说，我们将研究脂质过氧化在急性脑出血中的作用。 由于暴露在有毒氧气的条件下而造成的肺损伤 物种大概是形成的。因为还没有证明 脂质过氧化是致病机制中的必要步骤 肺损伤，我们建议确定脂质过氧化是否是必要的 导致肺损伤的事件或仅平行的化学过程。二 将对模型系统进行检查。第一个涉及肺损伤，导致 死于高氧症。第二项是佛波醇对肺损伤的研究。 肉豆蔻酸酯醋酸酯。我们将寻找过氧化产物的形成 这可以直接追溯到已知的化学反应。我们将尝试 来确定是什么化学物种引发了 过氧化。在本研究中，我们将使用气相色谱-质谱法 光谱技术，使我们能够量化分钟(皮克) 材料的数量。

Toxic oxygen species are implicated in causing lung injury. Evidence that oxy-radicals and other oxygen species are involved is inferential, but based on current understanding of oxygen chemistry in complex biological systems. It is important to understand how toxic oxygen species can cause tissue injury and by what chemical mechanisms they act. In general, we will investigate the role of lipid peroxidation in acute lung injury that results from exposure to conditions in which toxic oxygen species are presumably formed. Since it has not been demonstrated that lipid peroxidation is a necessary step in the mechanisms responsible for lung injury, we propose to determine if lipid peroxidation is an essential event leading to lung damage or only a parallel chemical process. Two model systems will be examined. The first involves lung damage resulting from hyperoxia. The second is a study of lung damage caused by phorbol myristate acetate. We will look for the formation of peroxidation products that can be directly traced to known chemical reactions. We will attempt to determine what chemical species are responsible for the initiation of peroxidation. In this study, we will use gas chromatography-mass spectrometric techniques that will allow us to quantitate minute (picogram) amounts of material.